Q5HZ60 · MCES2_ARATH
- ProteinmRNA cap guanine-N(7) methyltransferase 2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids354 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
mRNA capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity).
Catalytic activity
- a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 21 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Site | 48 | mRNA cap binding | |||
Binding site | 61 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 88-89 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Site | 278 | mRNA cap binding | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity | |
Molecular Function | RNA binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namemRNA cap guanine-N(7) methyltransferase 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ5HZ60
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000260152 | 1-354 | mRNA cap guanine-N7 methyltransferase 2 | ||
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 8-286 | mRNA cap 0 methyltransferase | |||
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q5HZ60-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length354
- Mass (Da)39,889
- Last updated2005-02-15 v1
- ChecksumD6950C807B3966D5
Q5HZ60-2
- Name2
- NoteMay be due to a competing acceptor splice site.
- Differences from canonical
- 60-60: I → IV
Q5HZ60-3
- Name3
- NoteMay be due to a competing acceptor splice site.
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_021576 | 60 | in isoform 2 | ||
Sequence conflict | 83 | in Ref. 3; BAD43168 | |||
Alternative sequence | VSP_021577 | 246-250 | in isoform 3 | ||
Alternative sequence | VSP_021578 | 251-354 | in isoform 3 | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL049711 EMBL· GenBank· DDBJ | CAB41341.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL132972 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002686 EMBL· GenBank· DDBJ | AEE78914.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE78915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE78916.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK175405 EMBL· GenBank· DDBJ | BAD43168.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176609 EMBL· GenBank· DDBJ | BAD44372.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176627 EMBL· GenBank· DDBJ | BAD44390.1 EMBL· GenBank· DDBJ | mRNA | ||
BT020464 EMBL· GenBank· DDBJ | AAW38965.1 EMBL· GenBank· DDBJ | mRNA | ||
BT020571 EMBL· GenBank· DDBJ | AAW78590.1 EMBL· GenBank· DDBJ | mRNA |