Q5HPI6 · PARE_STAEQ

Function

function

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.

Features

Showing features for binding site, site.

166450100150200250300350400450500550600650
Type
IDPosition(s)Description
Binding site7ATP (UniProtKB | ChEBI)
Binding site47ATP (UniProtKB | ChEBI)
Binding site74ATP (UniProtKB | ChEBI)
Binding site114-120ATP (UniProtKB | ChEBI)
Binding site341ATP (UniProtKB | ChEBI)
Binding site430Mg2+ 1 (UniProtKB | ChEBI); catalytic
Site455Interaction with DNA
Site458Interaction with DNA
Binding site503Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site503Mg2+ 2 (UniProtKB | ChEBI)
Binding site505Mg2+ 2 (UniProtKB | ChEBI)
Site510Interaction with DNA
Site626Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Molecular Functionmetal ion binding
Biological Processchromosome segregation
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 4 subunit B
  • EC number
  • Alternative names
    • Topoisomerase IV subunit B

Gene names

    • Name
      parE
    • Ordered locus names
      SERP0925

Organism names

Accessions

  • Primary accession
    Q5HPI6

Proteomes

Subcellular Location

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001454421-664DNA topoisomerase 4 subunit B

Interaction

Subunit

Heterotetramer composed of ParC and ParE.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

Type
IDPosition(s)Description
Compositional bias386-408Basic and acidic residues
Region386-418Disordered
Domain424-538Toprim

Sequence similarities

Belongs to the type II topoisomerase family. ParE type 2 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    664
  • Mass (Da)
    74,470
  • Last updated
    2005-12-20 v2
  • Checksum
    D2D3DF55FB629CAD
MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEVVDNSVDEVLNGYGDAITVTINQDGSISIEDNGRGMPTGIHASGKPTAEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYTQNFKNGGIPATGLVKTGKTKKTGTKVTFKPDSEIFKSTTTFNFDILSERLQESAFLLKDLKITLTDLRSGKEREEIYHYEEGIKEFVSYVNEGKEVLHDVTTFAGHSNGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNEYARRINELKDKDKNLDGNDIREGLTAIISVRIPEELLQFEGQTKSKLGTSEARSAVDSVVSEKLPYYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRIIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKNKKVEYAWTDEELENLQKQLGKGFILQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVAPRREWIEKHVEFGMQEDQSILDNKEVQILENEKYIEEETN

Sequence caution

The sequence AAW54307.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias386-408Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000029
EMBL· GenBank· DDBJ
AAW54307.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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