Q5HPI6 · PARE_STAEQ
- ProteinDNA topoisomerase 4 subunit B
- GeneparE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids664 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )
Note: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7 | ATP (UniProtKB | ChEBI) | |||
Binding site | 47 | ATP (UniProtKB | ChEBI) | |||
Binding site | 74 | ATP (UniProtKB | ChEBI) | |||
Binding site | 114-120 | ATP (UniProtKB | ChEBI) | |||
Binding site | 341 | ATP (UniProtKB | ChEBI) | |||
Binding site | 430 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | |||
Site | 455 | Interaction with DNA | |||
Site | 458 | Interaction with DNA | |||
Binding site | 503 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 503 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 505 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Site | 510 | Interaction with DNA | |||
Site | 626 | Interaction with DNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity | |
Molecular Function | metal ion binding | |
Biological Process | chromosome segregation | |
Biological Process | DNA topological change |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA topoisomerase 4 subunit B
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ5HPI6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000145442 | 1-664 | DNA topoisomerase 4 subunit B | ||
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 386-408 | Basic and acidic residues | |||
Region | 386-418 | Disordered | |||
Domain | 424-538 | Toprim | |||
Sequence similarities
Belongs to the type II topoisomerase family. ParE type 2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length664
- Mass (Da)74,470
- Last updated2005-12-20 v2
- ChecksumD2D3DF55FB629CAD
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 386-408 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000029 EMBL· GenBank· DDBJ | AAW54307.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |