Q5HCG4 · DNAJ_EHRRW

Function

function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per monomer.

Features

Showing features for binding site.

138250100150200250300350
TypeIDPosition(s)Description
Binding site151Zn2+ 1 (UniProtKB | ChEBI)
Binding site154Zn2+ 1 (UniProtKB | ChEBI)
Binding site168Zn2+ 2 (UniProtKB | ChEBI)
Binding site171Zn2+ 2 (UniProtKB | ChEBI)
Binding site190Zn2+ 2 (UniProtKB | ChEBI)
Binding site193Zn2+ 2 (UniProtKB | ChEBI)
Binding site204Zn2+ 1 (UniProtKB | ChEBI)
Binding site207Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionheat shock protein binding
Molecular Functionunfolded protein binding
Molecular Functionzinc ion binding
Biological Processchaperone cofactor-dependent protein refolding
Biological ProcessDNA replication
Biological Processprotein refolding
Biological Processresponse to heat

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Chaperone protein DnaJ

Gene names

    • Name
      dnaJ
    • Ordered locus names
      Erum0130, ERWE_CDS_09580

Organism names

Accessions

  • Primary accession
    Q5HCG4
  • Secondary accessions
    • Q5FCE1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000707821-382Chaperone protein DnaJ

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Features

Showing features for domain, zinc finger, repeat.

Type
IDPosition(s)Description
Domain5-70J
Zinc finger138-216CR-type
Repeat151-158CXXCXGXG motif
Repeat168-175CXXCXGXG motif
Repeat190-197CXXCXGXG motif
Repeat204-211CXXCXGXG motif

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Sequence similarities

Belongs to the DnaJ family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    382
  • Mass (Da)
    42,200
  • Last updated
    2005-02-15 v1
  • Checksum
    D1D5237F0E120C68
MSKSDYYDLLGLSKNATPEEIKKAYRKMALKYHPDKNPGDKAAEEKFKELSEAYDVLIDKDKRAAYDRYGHSAFSDGSGRGGFDFNSGFSTDFSDIFNDLFGGGFRGGRSSSKRQDGGTVGSDLRLDIEITLEDSFNGTKVPINYVTHVKCSSCSGSGSEGSVKSVQCNTCHGAGNIRTQQGFFTIERTCHVCNGEGEIIKNKCKKCSGSGRVRDEVNLLVTVPKGIESGDKIRLNGKGEAGYRGAQSGDLYVYPNIKKHKFFTRNGADLYCNVPIKMILATLGGHIEMPSIDGTWTKVKVPEGSQNGDKLRLKEKGMPVINSSRRGDMYIQITVETPINLTKQQKELLKKFDEEPNTVECNPQSTGFFQKVKSFWNDIRSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR767821
EMBL· GenBank· DDBJ
CAH57720.1
EMBL· GenBank· DDBJ
Genomic DNA
CR925678
EMBL· GenBank· DDBJ
CAI27452.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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