Q5HBC3 · TSAD_EHRRW
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids348 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- L-threonylcarbamoyladenylate + adenosine37 in tRNA = N6-L-threonylcarbamoyladenosine37 in tRNA + AMP + H+
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 116 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 120 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 138-142 | substrate | |||
Binding site | 171 | substrate | |||
Binding site | 184 | substrate | |||
Binding site | 282 | substrate | |||
Binding site | 310 | Fe cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rickettsiales > Anaplasmataceae > Ehrlichia
Accessions
- Primary accessionQ5HBC3
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000303355 | 1-348 | tRNA N6-adenosine threonylcarbamoyltransferase | ||
Structure
Sequence
- Sequence statusComplete
- Length348
- Mass (Da)38,012
- Last updated2005-02-15 v1
- Checksum10166F5EB21CECF4
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR767821 EMBL· GenBank· DDBJ | CAH58128.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CR925678 EMBL· GenBank· DDBJ | CAI26913.1 EMBL· GenBank· DDBJ | Genomic DNA |