Q5HBC3 · TSAD_EHRRW

Function

function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site116Fe cation (UniProtKB | ChEBI)
Binding site120Fe cation (UniProtKB | ChEBI)
Binding site138-142substrate
Binding site171substrate
Binding site184substrate
Binding site282substrate
Binding site310Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functioniron ion binding
Molecular FunctionN(6)-L-threonylcarbamoyladenine synthase activity
Biological ProcesstRNA threonylcarbamoyladenosine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA N6-adenosine threonylcarbamoyltransferase
  • EC number
  • Alternative names
    • N6-L-threonylcarbamoyladenine synthase
      (t(6)A synthase
      )
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
    • tRNA threonylcarbamoyladenosine biosynthesis protein TsaD

Gene names

    • Name
      tsaD
    • Synonyms
      gcp
    • Ordered locus names
      Erum4060, ERWE_CDS_04190

Organism names

Accessions

  • Primary accession
    Q5HBC3
  • Secondary accessions
    • Q5FEY4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003033551-348tRNA N6-adenosine threonylcarbamoyltransferase

Structure

Family & Domains

Sequence similarities

Belongs to the KAE1 / TsaD family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    348
  • Mass (Da)
    38,012
  • Last updated
    2005-02-15 v1
  • Checksum
    10166F5EB21CECF4
MVSNLLKVVLAIETSCDETAVAVVRSDKQVLSHKVLSQKEHVVYGGVVPEIASRAHINYLYDLTSQSIEESGCDLADIDAIAVTSGPGLIGGLIIGVMMAKAISSVTNKPIIEVNHLEAHTLLIRMFHDIDFPFLVLIISGGHCQFLIVHDVGCYQRLGSSLDDSLGEVFDKVARMLNLGYPGGPIIEQKSIMGDSKSFFLPRALINRFGCDFSFSGIKTAVRNIVVNQKYIDNDFICNISASFQDCIGDILVNRITNAIHMSQAINCKINKLVVTGGVAANHLLRNRISICVKDNNFEVLYPPTELCTDNGIMVGWAGIENLSKGYVSNLDFAPKARWPLESIKRSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR767821
EMBL· GenBank· DDBJ
CAH58128.1
EMBL· GenBank· DDBJ
Genomic DNA
CR925678
EMBL· GenBank· DDBJ
CAI26913.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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