Q5FR18 · THYX_GLUOX

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site89FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site109-112dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site112-114FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site120FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site120-124dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site195dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site211-213FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site217FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Active site222Involved in ionization of N3 of dUMP, leading to its activation
Binding site222dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionNADPH binding
Molecular Functionthymidylate synthase (FAD) activity
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-dependent thymidylate synthase
  • EC number
  • Short names
    FDTS
  • Alternative names
    • FAD-dependent thymidylate synthase
    • Thymidylate synthase ThyX
      (TS
      ; TSase
      )

Gene names

    • Name
      thyX
    • Ordered locus names
      GOX1427

Organism names

Accessions

  • Primary accession
    Q5FR18

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001755651-303Flavin-dependent thymidylate synthase

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain, motif.

Type
IDPosition(s)Description
Region1-21Disordered
Domain43-256ThyX
Motif112-122ThyX motif

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    303
  • Mass (Da)
    34,609
  • Last updated
    2005-03-01 v1
  • Checksum
    98E4392721F9DA72
MALTSEQRAEIEAQRSEPQLTSRPTVAAMENLLFTPFEVLDHGFLRVVDYMGDDGAVVQAARVSYGRGTKKVSEDAGLIRYLMRHRHSTPFEMCEIKFHVKLPVFVARQWIRHRMASVNEYSARYSILDREFYLPAMDQVAAQSSSNRQGRSDALDADTAHQVLEILRRDASQCYDDYESLLNPEGRGLARELARINLTLNTYTQWYWKIDLHNLMHFLALRADPHAQYEIRVYAEKMIEILKAWVPATAAAFEEYRLGAFTLSAGMLKVVRRRLAGETVTQENSGLTKREWSEMAAVLDGAS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000009
EMBL· GenBank· DDBJ
AAW61178.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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