Q5F882 · FITB_NEIG1

Function

function

Toxic component of a type II toxin-antitoxin (TA) system. Plays a role in the speed with which bacteria traverse human epithelial cells; disruption of the locus increases the speed of trafficking about 2-4-fold. FitAB binds to its own promoter better than FitA alone. The expected nuclease activity was not observed for the FitAB complex, perhaps because FitA (the antitoxin) prevents metal binding and thus catalysis by FitB.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

1139102030405060708090100110120130
TypeIDPosition(s)Description
Binding site5Mg2+ (UniProtKB | ChEBI)
Binding site104Mg2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Molecular FunctionRNA nuclease activity
Biological Processmigration in host

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Toxin FitB
  • Alternative names
    • Ribonuclease FitB (RNase FitB) (EC:3.1.-.-
      ) . EC:3.1.-.- (UniProtKB | ENZYME | Rhea)
    • Trafficking protein B

Gene names

    • Name
      fitB
    • Synonyms
      vapC
    • Ordered locus names
      NGO0907

Organism names

Accessions

  • Primary accession
    Q5F882
  • Secondary accessions
    • Q9RF91

Proteomes

Phenotypes & Variants

Disruption phenotype

Disruption of the fitAB locus leads to faster transepithelial cell trafficking of the bacterium; mutants adhere to and invade cells normally. Mutants grow normally in liquid culture but much faster within human cell lines A431 and T84; these latter 2 phenotypes were observed using MS11A bacteria with a disrupted fitAB locus.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004079001-139Toxin FitB

Interaction

Subunit

Forms a heterodimer with FitA, 4 FitAB heterodimers form a complex that binds to promoter DNA. The complex is also seen in solution. This protein does not actually contact DNA.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-123PINc

Sequence similarities

Belongs to the PINc/VapC protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    139
  • Mass (Da)
    15,307
  • Last updated
    2005-03-15 v1
  • Checksum
    AE12B8DF0E8427F4
MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELRLGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAAADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF200716
EMBL· GenBank· DDBJ
AAF19189.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004969
EMBL· GenBank· DDBJ
AAW89605.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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