Q5F7I3 · ARGJ_NEIG1

Function

function

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N2-acetylornithine and glutamate.

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N2-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N2-acetyl-L-ornithine from L-glutamate: step 1/4.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site119Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Site120Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Binding site152substrate
Binding site179substrate
Site189-190Cleavage; by autolysis
Active site190Nucleophile
Binding site190substrate
Binding site277substrate
Binding site401substrate
Binding site406substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionacetyl-CoA:L-glutamate N-acetyltransferase activity
Molecular Functionglutamate N-acetyltransferase activity
Molecular Functionmethione N-acyltransferase activity
Biological Processarginine biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Including 2 domains:

  • Recommended name
    Glutamate N-acetyltransferase
  • EC number
  • Alternative names
    • Ornithine acetyltransferase
      (OATase
      )
    • Ornithine transacetylase
  • Recommended name
    Amino-acid acetyltransferase
  • EC number
  • Alternative names
    • N-acetylglutamate synthase
      (AGSase
      )

Gene names

    • Name
      argJ
    • Ordered locus names
      NGO1194

Organism names

Accessions

  • Primary accession
    Q5F7I3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002272341-189Arginine biosynthesis bifunctional protein ArgJ alpha chain
ChainPRO_0000227235190-406Arginine biosynthesis bifunctional protein ArgJ beta chain

Keywords

Interaction

Subunit

Heterotetramer of two alpha and two beta chains.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the ArgJ family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    406
  • Mass (Da)
    42,851
  • Last updated
    2005-03-15 v1
  • Checksum
    A3F3635905E2C856
MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAGSTVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAAAARQIGCKPNQVMPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAASREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETFNTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDGEGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVADLDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCDLSHGYVSINADYRS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE004969
EMBL· GenBank· DDBJ
AAW89854.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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