Q5CCK3 · C90B2_ORYSJ

Function

function

Catalyzes the C22-alpha-hydroxylation step in brassinosteroid biosynthesis, which is the rate-limiting step in this biosynthetic pathway (PubMed:16369540).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxycathasterone (6-deoxoCT) (PubMed:16369540).
Required for auxin responses involved in the regulation of epidermal cells length of the lamina joint (PubMed:19605414).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Biotechnology

Null mutant plants for CYP90B1/DWARF4 produce increased biomass and grain yield at high density planting.

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site449Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmembrane
Molecular Functionfatty acid alpha-hydroxylase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Molecular Functionsteroid 22-alpha hydroxylase activity
Biological Processbrassinosteroid biosynthetic process
Biological Processbrassinosteroid homeostasis
Biological Processjasmonic acid mediated signaling pathway
Biological Processleaf development
Biological Processleaf shaping
Biological Processresponse to brassinosteroid
Biological Processsterol metabolic process
Biological Processunidimensional cell growth

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Steroid (22S)-hydroxylase
  • EC number
  • Alternative names
    • (22S)-22-hydroxycampesterol synthase
    • 6-deoxycathasterone synthase
    • Cytochrome P450 90B2
      (OsCYP90B2
      )
    • Protein DWARF4 homolog
      (OsDWARF4
      )
    • Steroid 22-alpha-hydroxylase

Gene names

    • Name
      CYP90B2
    • Synonyms
      CYP90B1
      , DWARF4
    • ORF names
      OJ1626B05.9
    • Ordered locus names
      Os03g0227700
      , LOC_Os03g12660

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q5CCK3
  • Secondary accessions
    • Q8H848

Proteomes

Genome annotation databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane12-32Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Slight dwarf phenotype and erect leaves (PubMed:16369540).
Impaired response to auxin leading to reduced epidermal cells length of the lamina joint (PubMed:19605414).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50089702681-506Steroid (22S)-hydroxylase

Proteomic databases

Expression

Tissue specificity

Highly expressed in roots and leaf blades. Expressed in shoot apex, stems, leaf sheaths, inflorescences and flowers.

Induction

Down-regulated by brassinolide (BL).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    57,329
  • Last updated
    2005-04-12 v1
  • Checksum
    68E733DA80556B9F
MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGTTPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE

Sequence caution

The sequence AAN60994.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB206579
EMBL· GenBank· DDBJ
BAD90972.1
EMBL· GenBank· DDBJ
mRNA
AC104473
EMBL· GenBank· DDBJ
AAN60994.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
DP000009
EMBL· GenBank· DDBJ
ABF94760.1
EMBL· GenBank· DDBJ
Genomic DNA
AP008209
EMBL· GenBank· DDBJ
BAF11361.2
EMBL· GenBank· DDBJ
Genomic DNA
AP014959
EMBL· GenBank· DDBJ
BAS83075.1
EMBL· GenBank· DDBJ
Genomic DNA
AK243298
EMBL· GenBank· DDBJ
BAH01529.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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