Q5CCK3 · C90B2_ORYSJ
- ProteinSteroid (22S)-hydroxylase
- GeneCYP90B2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids506 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the C22-alpha-hydroxylation step in brassinosteroid biosynthesis, which is the rate-limiting step in this biosynthetic pathway (PubMed:16369540).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxycathasterone (6-deoxoCT) (PubMed:16369540).
Required for auxin responses involved in the regulation of epidermal cells length of the lamina joint (PubMed:19605414).
Catalyzes the conversion of campesterol (CR) to (22S)-22-hydroxycampesterol (22-OHCR, 22-hydroxyCR) and of campestanol (CN) to 6-deoxycathasterone (6-deoxoCT) (PubMed:16369540).
Required for auxin responses involved in the regulation of epidermal cells length of the lamina joint (PubMed:19605414).
Catalytic activity
- a C28-steroid + O2 + reduced [NADPH--hemoprotein reductase] = a (22S)-22-hydroxy C28-steroid + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- campesterol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycampesterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- campestanol + O2 + reduced [NADPH--hemoprotein reductase] = 6-deoxycathasterone + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Biotechnology
Null mutant plants for CYP90B1/DWARF4 produce increased biomass and grain yield at high density planting.
Pathway
Plant hormone biosynthesis; brassinosteroid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | fatty acid alpha-hydroxylase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Molecular Function | steroid 22-alpha hydroxylase activity | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | brassinosteroid homeostasis | |
Biological Process | jasmonic acid mediated signaling pathway | |
Biological Process | leaf development | |
Biological Process | leaf shaping | |
Biological Process | response to brassinosteroid | |
Biological Process | sterol metabolic process | |
Biological Process | unidimensional cell growth |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSteroid (22S)-hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ5CCK3
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 12-32 | Helical | ||||
Sequence: LLFFLPFILLALLTFYTTTVA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5008970268 | 1-506 | Steroid (22S)-hydroxylase | |||
Sequence: MAAMMASITSELLFFLPFILLALLTFYTTTVAKCHGGHWWRGGTTPAKRKRMNLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPIRVHRIAQDDEQE |
Proteomic databases
Expression
Tissue specificity
Highly expressed in roots and leaf blades. Expressed in shoot apex, stems, leaf sheaths, inflorescences and flowers.
Induction
Down-regulated by brassinolide (BL).
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)57,329
- Last updated2005-04-12 v1
- Checksum68E733DA80556B9F
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB206579 EMBL· GenBank· DDBJ | BAD90972.1 EMBL· GenBank· DDBJ | mRNA | ||
AC104473 EMBL· GenBank· DDBJ | AAN60994.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
DP000009 EMBL· GenBank· DDBJ | ABF94760.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008209 EMBL· GenBank· DDBJ | BAF11361.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014959 EMBL· GenBank· DDBJ | BAS83075.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK243298 EMBL· GenBank· DDBJ | BAH01529.1 EMBL· GenBank· DDBJ | mRNA |