Q5CB03 · B3GA1_CANLF
- ProteinGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
- GeneB3GAT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids335 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Catalytic activity
- 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + H+ + UDP
Cofactor
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 92-94 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: PTY | ||||||
Binding site | 123 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 166 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 171 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 196-198 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: DDD | ||||||
Binding site | 198 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 229 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: E | ||||||
Active site | 285 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Binding site | 312-314 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | ||||
Sequence: HTR | ||||||
Site | 322 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | extracellular region | |
Cellular Component | Golgi membrane | |
Molecular Function | galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | carbohydrate metabolic process | |
Biological Process | chondroitin sulfate proteoglycan biosynthetic process | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Canidae > Canis
Accessions
- Primary accessionQ5CB03
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MPKRRD | ||||||
Transmembrane | 7-27 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: ILAIVLIVLPWTLLVTVWHQS | ||||||
Topological domain | 28-335 | Lumenal | ||||
Sequence: TIAPLLTTHKGEPLTDSRREAAPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPTVEI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000195166 | 1-335 | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 | |||
Sequence: MPKRRDILAIVLIVLPWTLLVTVWHQSTIAPLLTTHKGEPLTDSRREAAPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPTVEI | ||||||
Modified residue | 104 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 109 | Phosphothreonine | ||||
Sequence: T | ||||||
Glycosylation | 141 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 185 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 304 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
The soluble form derives from the membrane form by proteolytic processing.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 3-5 | Essential for transport from endoplasmic reticulum to Golgi apparatus and interaction with SAR1A | ||||
Sequence: KRR | ||||||
Region | 246-255 | Interaction with galactose moiety of substrate glycoprotein | ||||
Sequence: FDPHRPFAID |
Sequence similarities
Belongs to the glycosyltransferase 43 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length335
- Mass (Da)38,361
- Last updated2005-04-12 v1
- Checksum194B8FE05DBE4864
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ888975 EMBL· GenBank· DDBJ | CAI62040.1 EMBL· GenBank· DDBJ | mRNA |