Q5CB03 · B3GA1_CANLF

Function

function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Protein modification; protein glycosylation.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site92-94UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site123UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site166UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site171UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site196-198UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site198Mn2+ (UniProtKB | ChEBI)
Site229Interaction with galactose moiety of substrate glycoprotein
Active site285Proton donor/acceptor
Binding site312-314UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Site322Interaction with galactose moiety of substrate glycoprotein

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentextracellular region
Cellular ComponentGolgi membrane
Molecular Functiongalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
Molecular Functionmetal ion binding
Biological Processcarbohydrate metabolic process
Biological Processchondroitin sulfate proteoglycan biosynthetic process
Biological Processprotein glycosylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
  • EC number
  • Alternative names
    • Beta-1,3-glucuronyltransferase 1
    • Glucuronosyltransferase P (GlcAT-P
      )
    • UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase (GlcUAT-P)

Gene names

    • Name
      B3GAT1

Organism names

Accessions

  • Primary accession
    Q5CB03

Proteomes

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-6Cytoplasmic
Transmembrane7-27Helical; Signal-anchor for type II membrane protein
Topological domain28-335Lumenal

Keywords

PTM/Processing

Features

Showing features for chain, modified residue, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00001951661-335Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Modified residue104Phosphothreonine
Modified residue109Phosphothreonine
Glycosylation141N-linked (GlcNAc...) asparagine
Glycosylation185N-linked (GlcNAc...) asparagine
Glycosylation304N-linked (GlcNAc...) asparagine

Post-translational modification

The soluble form derives from the membrane form by proteolytic processing.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Homodimer. Interacts with SAR1A.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region3-5Essential for transport from endoplasmic reticulum to Golgi apparatus and interaction with SAR1A
Region246-255Interaction with galactose moiety of substrate glycoprotein

Sequence similarities

Belongs to the glycosyltransferase 43 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    38,361
  • Last updated
    2005-04-12 v1
  • Checksum
    194B8FE05DBE4864
MPKRRDILAIVLIVLPWTLLVTVWHQSTIAPLLTTHKGEPLTDSRREAAPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPTVEI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ888975
EMBL· GenBank· DDBJ
CAI62040.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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