Q5BE65 · KDSR_EMENI
- Protein3-ketodihydrosphingosine reductase tsc10
- Genetsc10
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids369 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.
Catalytic activity
- sphinganine + NADP+ = 3-oxosphinganine + NADPH + H+This reaction proceeds in the backward direction.
Pathway
Lipid metabolism; sphingolipid metabolism.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 52 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 55 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 57 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 59 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 80 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 84 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 110 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 110 | NADPH (UniProtKB | ChEBI) | |||
Binding site | 111 | NADPH (UniProtKB | ChEBI) | |||
Active site | 213 | Proton acceptor | |||
Binding site | 213 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 217 | Lowers pKa of active site Tyr | |||
Binding site | 217 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 261 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 263 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 272 | NADP+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | 3-dehydrosphinganine reductase activity | |
Molecular Function | NADPH binding | |
Biological Process | 3-keto-sphinganine metabolic process | |
Biological Process | sphingolipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-ketodihydrosphingosine reductase tsc10
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5BE65
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 195-215 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000054796 | 1-369 | 3-ketodihydrosphingosine reductase tsc10 | ||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 55-59 | GXSXG | |||
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)40,461
- Last updated2011-11-16 v2
- MD5 Checksum6123B4CCD34B47BCAC08097D00F6301A
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AACD01000016 EMBL· GenBank· DDBJ | EAA66283.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BN001308 EMBL· GenBank· DDBJ | CBF88015.1 EMBL· GenBank· DDBJ | Genomic DNA |