Q5BA93 · PLYL_EMENI
- ProteinPectate lyase PEL9
- GenePEL9
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids410 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Presents an endo-cleaving activity on the homogalacturonan (HG) region in pectin (PubMed:35802235).
Active on homogalacturonan with a degree of polymerization above 4, and does not appear to be affected by the degree of methylation of the substrate (PubMed:35802235).
Does not degrade linear rhamnogalacturonan (PubMed:35802235).
Active on homogalacturonan with a degree of polymerization above 4, and does not appear to be affected by the degree of methylation of the substrate (PubMed:35802235).
Does not degrade linear rhamnogalacturonan (PubMed:35802235).
Catalytic activity
Cofactor
Activity regulation
Inhibited by iron ions (PubMed:35802235).
Activated in presence of the surfactant polysorbate 20, while inhibited in the presence of Triton X-100 and sodium dodecyl sulfate (PubMed:35802235).
Inhibited in presence of the organic solvents methanol, ethanol, propan-2-ol and acetone (PubMed:35802235).
Activated in presence of the surfactant polysorbate 20, while inhibited in the presence of Triton X-100 and sodium dodecyl sulfate (PubMed:35802235).
Inhibited in presence of the organic solvents methanol, ethanol, propan-2-ol and acetone (PubMed:35802235).
Kinetics
kcat is 280 sec-1 for homogalacturonan (HG) (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 357 sec-1 for apple pectin (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 272 sec-1 for citrus peel pectin (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 236 sec-1 for rhamnogalacturonan type I (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 357 sec-1 for apple pectin (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 272 sec-1 for citrus peel pectin (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
kcat is 236 sec-1 for rhamnogalacturonan type I (at 45 degrees Celsius and at pH 8) (PubMed:35802235).
pH Dependence
Optimum pH is 8.
Temperature Dependence
Optimum temperature is 45 degrees Celsius.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | carbon-oxygen lyase activity, acting on polysaccharides | |
Molecular Function | metal ion binding | |
Molecular Function | pectate lyase activity | |
Biological Process | symbiont-mediated disruption of host cell wall |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePectate lyase PEL9
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5BA93
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-18 | |||||
Sequence: MMGKSVWVFAALFPAVLA | ||||||
Chain | PRO_5010168540 | 19-410 | Pectate lyase PEL9 | |||
Sequence: ADIYVSPDGSDDAAGTIDAPLQSIQLAVDQATAGSTIYLRGGTYTPTSNIQITKSGTASAPYVLRAYEGESVIIDGEELPGTPADLDASLDNADRGILHIQDAEYWEFYDLELINGPYGVYARDASNNHYERITTRNNYETGFQLQGESSNNVVLYLDSYGNRDPRKNGESADGFACKEGSGEGNILRGARLWNNVDDGLDLWYAVNPVHPRISANTSREFKSAVTIEDTIAWGNGFNRWDFTPFEGDGNGFKLGGGDDTDIGPADHIITNCIAFSNAKDGFTDNSQPGNFVLTRNTAWDNTAVGFKFGTAVATLTGNIAASNGEAPTSLSDEQISDGNSWDGDEDWDDGSFVSVDVSLVQGERNADGTIEPSGFLLPADGEEIGATTDWSA | ||||||
Glycosylation | 234 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Expression
Induction
Induced during growth on homogalacturonan (HG).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 342-361 | Disordered | ||||
Sequence: GEAPTSLSDEQISDGNSWDG | ||||||
Region | 381-410 | Disordered | ||||
Sequence: ERNADGTIEPSGFLLPADGEEIGATTDWSA |
Sequence similarities
Belongs to the polysaccharide lyase 9 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length410
- Mass (Da)43,891
- Last updated2005-04-26 v1
- Checksum1BF619F86AB51CE6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BN001307 EMBL· GenBank· DDBJ | CBF87052.1 EMBL· GenBank· DDBJ | Genomic DNA |