Q5B833 · MANB_EMENI
- ProteinMannan endo-1,4-beta-mannosidase B
- GenemanB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids387 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780).
Also has efficient transglycosylation activity. Produces mainly mannopentaose and mannoheptaose out of mannotetraose. May even prefer sugars as acceptors instead of water (PubMed:24950755).
Also has efficient transglycosylation activity. Produces mainly mannopentaose and mannoheptaose out of mannotetraose. May even prefer sugars as acceptors instead of water (PubMed:24950755).
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.6 mg/ml | locust bean gum mannan |
pH Dependence
Optimum pH is 6.
Temperature Dependence
Optimum temperature is 60 degrees Celsius.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 86 | substrate | ||||
Sequence: W | ||||||
Binding site | 203 | substrate | ||||
Sequence: N | ||||||
Active site | 204 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Binding site | 204-206 | substrate | ||||
Sequence: EPR | ||||||
Binding site | 240 | substrate | ||||
Sequence: E | ||||||
Binding site | 279 | substrate | ||||
Sequence: Y | ||||||
Binding site | 283 | substrate | ||||
Sequence: W | ||||||
Active site | 312 | Nucleophile | ||||
Sequence: E | ||||||
Binding site | 342 | substrate | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | mannan endo-1,4-beta-mannosidase activity | |
Biological Process | mannan catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMannan endo-1,4-beta-mannosidase B
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5B833
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MRLIHAVLGLLAGAAPALVAASPA | ||||||
Chain | PRO_0000393708 | 25-387 | Mannan endo-1,4-beta-mannosidase B | |||
Sequence: APIGNGRDQVSKAVGRHFEIDGKVQYFAGTNCWWLGNLLNDFEVELAVSQIAETGYKVVRTWGFFGVNDPSNPGQPVYYQVLNESLYEGGLGINYGSNGIRRLDTVVSLAERYDIQLVLTFMNNWNDFGGINIYSNAFGSNATTWYTDKKSQRAYREYIKFIVNRYKGSSAIFAWELGNEPRCKGCDPSVIYNWAKSVSAYIKKLDKKHMVALGDEGWLCPPEGDGTYAYDCSEGVDFVKNLEIETLDYGTFHLYPESWGYNYSWGSEWVLQHDAIGKRFNKPVVFEEYGTPLNHTQLERPWQLTTVKETQVAADFIWQFGTVLPVEGTEWGDVNSIYYGTEEYEVLAVQHAWEMARKKVPRH | ||||||
Glycosylation | 107 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 165 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 207↔210 | |||||
Sequence: CKGC | ||||||
Glycosylation | 286 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 318 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length387
- Mass (Da)43,725
- Last updated2010-04-20 v2
- Checksum8CC8A4C1253E7823
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ490485 EMBL· GenBank· DDBJ | ABF50861.1 EMBL· GenBank· DDBJ | mRNA | ||
AACD01000055 EMBL· GenBank· DDBJ | EAA63265.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BN001306 EMBL· GenBank· DDBJ | CBF83007.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |