Q5AZ53 · MANC_EMENI
- ProteinMannan endo-1,4-beta-mannosidase C
- GenemanC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids399 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780).
Also has transglycosylation activity. Produces mainly mannopentaose and mannohexaose out of mannotetraose (PubMed:21867780, PubMed:24950755).
Also has transglycosylation activity. Produces mainly mannopentaose and mannohexaose out of mannotetraose (PubMed:21867780, PubMed:24950755).
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.1 mg/ml | locust bean gum mannan | |||||
1.8 mM | mannopentaose | |||||
0.6 mM | mannohexaose |
pH Dependence
Optimum pH is 5.5.
Temperature Dependence
Optimum temperature is 50 degrees Celsius.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 74 | substrate | ||||
Sequence: W | ||||||
Binding site | 195 | substrate | ||||
Sequence: N | ||||||
Active site | 196 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Binding site | 196-198 | substrate | ||||
Sequence: EPR | ||||||
Binding site | 243 | substrate | ||||
Sequence: E | ||||||
Binding site | 280 | substrate | ||||
Sequence: Y | ||||||
Binding site | 284 | substrate | ||||
Sequence: W | ||||||
Active site | 313 | Nucleophile | ||||
Sequence: E | ||||||
Binding site | 355 | substrate | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | mannan endo-1,4-beta-mannosidase activity | |
Biological Process | mannan catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMannan endo-1,4-beta-mannosidase C
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5AZ53
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-18 | |||||
Sequence: MIFSTLLSLALLATTATA | ||||||
Chain | PRO_0000393710 | 19-399 | Mannan endo-1,4-beta-mannosidase C | |||
Sequence: RKGFVTTKGDKFQLDGKDFYFAGSNAYYFPFNNNQTDVELGLSAAKKAGLLVFRTWGFNDKNVTYIEDGLPQYGGEGAGTTEVVFQWWQNGTSTIDLEPFDKVVNAAAKTGIKLIVTLVNNWADYGGMDVYTVNLGGQYHDDFYRLPQIKKAYKRYVKEMVTRYRNSPAIMAWELANEPRCGADGVRNLPASDECTPELLTSWIDEMSTYVKRLDPHHLVTWGGEGGFNYDSDDWAYNGSDGGDFEAELKLKNIDFGVFHSYPDWWSKTVEWTNKWIVDHARAARRVGKPVVHEEYGWLTPQGRLDNLGTVSNITRLEAVGGWQSISLREKMSDMFWQFGYSGYSYGRNHDDGFTIYLDDAEAQELVYKHAKEVNKLNRRR | ||||||
Glycosylation | 52 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 80 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 108 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 199↔213 | |||||
Sequence: CGADGVRNLPASDEC | ||||||
Glycosylation | 256 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 331 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length399
- Mass (Da)45,338
- Last updated2005-04-26 v1
- Checksum9898F0BF644934CE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ490502 EMBL· GenBank· DDBJ | ABF50878.1 EMBL· GenBank· DDBJ | mRNA | ||
AACD01000108 EMBL· GenBank· DDBJ | EAA58449.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BN001301 EMBL· GenBank· DDBJ | CBF69494.1 EMBL· GenBank· DDBJ | Genomic DNA |