Q5AUY0 · DBAF_EMENI
- ProteinFAD-dependent oxidoreductase dbaF
- GenedbaF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids467 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
FAD-dependent oxidoreductase; part of the gene cluster that mediates the biosynthesis of the antibiotic 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA) and its derivatives (PubMed:22510154, PubMed:23001671).
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)
Cofactor
Pathway
Secondary metabolite biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFAD-dependent oxidoreductase dbaF
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5AUY0
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Reduces the amounts of DHMDA produced.
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-20 | |||||
Sequence: MKSVLASGALTLAFSLAALA | ||||||
Chain | PRO_5010288538 | 21-467 | FAD-dependent oxidoreductase dbaF | |||
Sequence: ADAFQASSWDSTHIIRRDVVIVGGGAAGTYAAIRLKDHGKSVVLVERRDRLGGHAVTYKDPNTGGSVDYGVQVYDNNTVVRDFFSRLNTPLADLSFASFGKPVYADFEEGMLLNLTAGTLGQDYINELNKYPYLDNGFELPDPVPEDLLLPWVEYIGKYNIDLSTAIATLARPAVTGNLLNILAIYVFNNLNHLLLHEMSGAVVVNANRDNSQLYRNAVSELQPDLLLRSRVVAGQRRTRKRDGVRLVVDTPTGRKLIIAKQLIVGMPPILDNMRTFGLDSHEHSVLSHIYGLPYYGGVVSDTGLAPGFSFKNYAANTSYNLAEIPSVVAFNPSSVDGLFYYWYNAPQPVSQRRIETEARDAIKTLQRLTNSTTQPEPKFLAFSDFAPYQLRVSAEAIRNGFYDDMYGLQGHRNTWYTGTLFVTGSSQVWNNTEVMLPEILAAVNSS | ||||||
Glycosylation | 96 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 134 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 337 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 391 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 451 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Induction
Deletion of the conserved eukaryotic csnE deneddylase subunit of the COP9 signalosome leading to defect in protein degradation results in the activation of the silenced dba gene cluster (PubMed:23001671).
Expression is positively regulated by the dba cluster specific transcription factors dbaA and dbaG (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).
Expression is positively regulated by the dba cluster specific transcription factors dbaA and dbaG (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length467
- Mass (Da)51,478
- Last updated2005-04-26 v1
- Checksum33086CC663EDE630
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AACD01000135 EMBL· GenBank· DDBJ | EAA59554.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BN001302 EMBL· GenBank· DDBJ | CBF73488.1 EMBL· GenBank· DDBJ | Genomic DNA |