Q5AUY0 · DBAF_EMENI

Function

function

FAD-dependent oxidoreductase; part of the gene cluster that mediates the biosynthesis of the antibiotic 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA) and its derivatives (PubMed:22510154, PubMed:23001671).
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Secondary metabolite biosynthesis.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functionoxidoreductase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent oxidoreductase dbaF
  • EC number
  • Alternative names
    • Derivative of benzaldehyde biosynthesis cluster protein F

Gene names

    • Name
      dbaF
    • ORF names
      ANIA_07900

Organism names

Accessions

  • Primary accession
    Q5AUY0
  • Secondary accessions
    • A0A1U8QSV1
    • C8V4K0

Proteomes

Phenotypes & Variants

Disruption phenotype

Reduces the amounts of DHMDA produced.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-20
ChainPRO_501028853821-467FAD-dependent oxidoreductase dbaF
Glycosylation96N-linked (GlcNAc...) asparagine
Glycosylation134N-linked (GlcNAc...) asparagine
Glycosylation337N-linked (GlcNAc...) asparagine
Glycosylation391N-linked (GlcNAc...) asparagine
Glycosylation451N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

Deletion of the conserved eukaryotic csnE deneddylase subunit of the COP9 signalosome leading to defect in protein degradation results in the activation of the silenced dba gene cluster (PubMed:23001671).
Expression is positively regulated by the dba cluster specific transcription factors dbaA and dbaG (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    467
  • Mass (Da)
    51,478
  • Last updated
    2005-04-26 v1
  • Checksum
    33086CC663EDE630
MKSVLASGALTLAFSLAALAADAFQASSWDSTHIIRRDVVIVGGGAAGTYAAIRLKDHGKSVVLVERRDRLGGHAVTYKDPNTGGSVDYGVQVYDNNTVVRDFFSRLNTPLADLSFASFGKPVYADFEEGMLLNLTAGTLGQDYINELNKYPYLDNGFELPDPVPEDLLLPWVEYIGKYNIDLSTAIATLARPAVTGNLLNILAIYVFNNLNHLLLHEMSGAVVVNANRDNSQLYRNAVSELQPDLLLRSRVVAGQRRTRKRDGVRLVVDTPTGRKLIIAKQLIVGMPPILDNMRTFGLDSHEHSVLSHIYGLPYYGGVVSDTGLAPGFSFKNYAANTSYNLAEIPSVVAFNPSSVDGLFYYWYNAPQPVSQRRIETEARDAIKTLQRLTNSTTQPEPKFLAFSDFAPYQLRVSAEAIRNGFYDDMYGLQGHRNTWYTGTLFVTGSSQVWNNTEVMLPEILAAVNSS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AACD01000135
EMBL· GenBank· DDBJ
EAA59554.1
EMBL· GenBank· DDBJ
Genomic DNA
BN001302
EMBL· GenBank· DDBJ
CBF73488.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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