Q5AUX7 · DBAI_EMENI
- ProteinNon-reducing polyketide synthase dbaI
- GenedbaI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2605 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the antibiotic 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA) and its derivatives (PubMed:22510154, PubMed:23001671).
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)
Catalytic activity
- 4 malonyl-CoA + acetyl-CoA + AH2 + S-adenosyl-L-methionine + 3 H+ = 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde + A + S-adenosyl-L-homocysteine + 4 CO2 + 5 CoA + H2OThis reaction proceeds in the forward direction.
4 CHEBI:57384 + CHEBI:57288 + CHEBI:17499 + CHEBI:59789 + 3 CHEBI:15378 = CHEBI:155860 + CHEBI:13193 + CHEBI:57856 + 4 CHEBI:16526 + 5 CHEBI:57287 + CHEBI:15377
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 144 | Nucleophile; for transacylase activity | |||
Active site | 262 | Proton donor/acceptor; for transacylase activity | |||
Active site | 547 | For beta-ketoacyl synthase activity | |||
Active site | 682 | For beta-ketoacyl synthase activity | |||
Active site | 721 | For beta-ketoacyl synthase activity | |||
Active site | 1320 | Proton acceptor; for dehydratase activity | |||
Active site | 1504 | Proton donor; for dehydratase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity | |
Molecular Function | methyltransferase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | methylation | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNon-reducing polyketide synthase dbaI
- EC number
- Short namesNR-PKS dbaI
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionQ5AUX7
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000446352 | 1-2605 | Non-reducing polyketide synthase dbaI | ||
Modified residue | 1699 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Expression
Induction
Deletion of the conserved eukaryotic csnE deneddylase subunit of the COP9 signalosome leading to defect in protein degradation results in the activation of the silenced dba gene cluster (PubMed:23001671).
Expression is positively regulated by the dba cluster specific transcription factor dbaA (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).
Expression is positively regulated by the dba cluster specific transcription factor dbaA (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 97-243 | N-terminal acylcarrier protein transacylase domain (SAT) | |||
Domain | 382-798 | Ketosynthase family 3 (KS3) | |||
Region | 908-1195 | Malonyl-CoA:ACP transacylase (MAT) domain | |||
Region | 1285-1420 | N-terminal hotdog fold | |||
Domain | 1285-1596 | PKS/mFAS DH | |||
Region | 1316-1594 | Product template (PT) domain | |||
Region | 1447-1596 | C-terminal hotdog fold | |||
Domain | 1665-1739 | Carrier | |||
Region | 1742-1780 | Disordered | |||
Compositional bias | 1745-1773 | Polar residues | |||
Region | 1963-2151 | Methyltransferase domain | |||
Region | 2230-2473 | NADPH-binding (R) domain | |||
Domain
Multidomain protein; including an N-terminal starter unit:ACP transacylase (SAT) domain, a beta-ketoacyl synthase (KS) domain, a malonyl-CoA:ACP transacylase (MAT) domain, a product template domain, a acyl carrier protein (ACP) domain, a methyltransferase domain and a reductive NADPH-binding domain that is required for NADPH-dependent product release.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,605
- Mass (Da)288,997
- Last updated2019-02-13 v2
- MD5 Checksum81C7B409751D85AE61977650C029A80F
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1745-1773 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AACD01000135 EMBL· GenBank· DDBJ | EAA59557.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BN001302 EMBL· GenBank· DDBJ | CBF73494.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |