Q5AUX7 · DBAI_EMENI

Function

function

Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the antibiotic 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA) and its derivatives (PubMed:22510154, PubMed:23001671).
The direct non-reducing polyketide synthase dbaI product is 2,4-dihydroxy-3-methyl-6-(2-oxopropyl)benzaldehyde (DHMBA), produced by condensation of one acetyl-CoA starter unit with 4 malonyl-CoA units and one methylation step (PubMed:22510154).
The FAD-dependent monooxygenase dbaH is responsible for the synthesis of yellow pigments derived from the oxidation of DHMBA (PubMed:23001671).
The roles of dbaB, C, E and F have still to be determined (Probable)

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site144Nucleophile; for transacylase activity
Active site262Proton donor/acceptor; for transacylase activity
Active site547For beta-ketoacyl synthase activity
Active site682For beta-ketoacyl synthase activity
Active site721For beta-ketoacyl synthase activity
Active site1320Proton acceptor; for dehydratase activity
Active site1504Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Functionacyltransferase activity
Molecular Functionmethyltransferase activity
Molecular Functionphosphopantetheine binding
Biological Processmethylation
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Non-reducing polyketide synthase dbaI
  • EC number
  • Short names
    NR-PKS dbaI
  • Alternative names
    • Derivative of benzaldehyde biosynthesis cluster protein I

Gene names

    • Name
      dbaI
    • Synonyms
      pkeA
    • ORF names
      ANIA_07903

Organism names

Accessions

  • Primary accession
    Q5AUX7
  • Secondary accessions
    • A0A1U8QHY2
    • C8V4K3

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00004463521-2605Non-reducing polyketide synthase dbaI
Modified residue1699O-(pantetheine 4'-phosphoryl)serine

Keywords

Expression

Induction

Deletion of the conserved eukaryotic csnE deneddylase subunit of the COP9 signalosome leading to defect in protein degradation results in the activation of the silenced dba gene cluster (PubMed:23001671).
Expression is positively regulated by the dba cluster specific transcription factor dbaA (PubMed:23001671).
Expression is also controlled by the transcription factor flbB (PubMed:25701285).

Structure

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region97-243N-terminal acylcarrier protein transacylase domain (SAT)
Domain382-798Ketosynthase family 3 (KS3)
Region908-1195Malonyl-CoA:ACP transacylase (MAT) domain
Region1285-1420N-terminal hotdog fold
Domain1285-1596PKS/mFAS DH
Region1316-1594Product template (PT) domain
Region1447-1596C-terminal hotdog fold
Domain1665-1739Carrier
Region1742-1780Disordered
Compositional bias1745-1773Polar residues
Region1963-2151Methyltransferase domain
Region2230-2473NADPH-binding (R) domain

Domain

Multidomain protein; including an N-terminal starter unit:ACP transacylase (SAT) domain, a beta-ketoacyl synthase (KS) domain, a malonyl-CoA:ACP transacylase (MAT) domain, a product template domain, a acyl carrier protein (ACP) domain, a methyltransferase domain and a reductive NADPH-binding domain that is required for NADPH-dependent product release.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,605
  • Mass (Da)
    288,997
  • Last updated
    2019-02-13 v2
  • MD5 Checksum
    81C7B409751D85AE61977650C029A80F
MLGHRDFTTLPLSRREFLLFGPLALSFDQAAFEHLRKTIVNSEEHRWALEVLGSLPQYYATIVNAFPGINGRNEVQLEDLKGALHSGKPLATSFPLPNTLLIPLVMVLHLTEYSRFLQEISEELESGIDLFDASRHNKETVGFCTGLLSAMAVSSAGSREDFRKYAAVAVRLGLLVGVVVDSHDISSAQGPSKSISASWNSAQKREDARRIMDEFPQAYISVYYDEDRATITAPASEISDLHRRLRASGIVTAEIGLNGCFHADCYLDQLDPIIQFCDSQPDFQLPDASKVVIPTRSNATGELIRDGALHQHALRSILVEPPQWFESFTAVRDACAEDEGAIIFSFGPERCVPPSLLRVLSQKVVTVEDLDVLKRYQYSYSENDIAVVGMSCKVAGANNLEEFWDLLCTGKSQHREVPKERFSFETVFRDVDSKRKWFGNFIDGHDQFDHKFFKKSPRESATMDPQQRHLLQIAYQAVEQSGYFHSANPDRQIGCYMGVCACDYENNIACHAPNAFSATGNLQGFIAGKVSHFFGWTGPGLTIDTACSSSAVAVHQACKAIITGECTAALAGGTHVMTNPLWFQNLAGASFLSTTGQCKPFDAKADGYCRGEGIATVFLKKLSAAVADGDQILGVITATAVQQNQNCTPIFVPNVPSLSDLFRVVVKQSRLQPSDVTVVEAHGTGTAVGDPAEYDSIRSVLGGSSREKTLALSSVKGLVGHIECTSGIVSLIKVLLMLQKRMIPPQASFTTINPAIKATPADKINIPTTVKTWDAEFCAALINNYGASGSNASIVVTQPPVGTVKPSAETSGLKYPFRFCGMDEQSLRRYSKIFRQFLNRKSYSAQDLSLRNISFNVNRQSNRQLDRTLLFSVKTLEELEQKLVTFENDNDSITSLALPKSKPVVLCFGGQVSTFVGLDRTVYERVAILRKHLHTVDAVARSIGLKSIFPRIFETTPVSDTVHLQIMLFASQYACARSWIDSGIQPVAVVGHSFGELTSLCVSQSLSLEDAVKMIAARATLIRDAWGPEKGAMLAVEADLEDVQKLLAESSAGCQDVQPATIACYNGPRSFTLAGAVAAIDAVAEALATPAFSSMKNKRLNVTNAFHCALVDPLLDRLEESARELTFRAPVIPVQRATEYQTEELPTSRFVADHIRSPVFFNHAIHRLADKYPSCVFLEAGSNSTVTNMASRALGNPSSSHFQAINITSHNGWNNLVDATMNMWKSGLGVHFWAHQPSQTKEYALLLLPPYQFEPSRHWIELKNPPKLTAAPAIEEVKKEEAKVPNTLLTFVGYQDSERQQARFRVNTMIPKYDKLIRGHIIAQTAPICPATVQLDLVIESIRSIRPELASTEHEPQIHAVENLAPICVNPLRAVWVEVTADDVAQGTSWNFQVYSDDLQNGFSKTIHTTGRVIFRSISDVSLKYEFARFERHFRHQTCVELMRGGEVDEVLQNRNIYKMFAEIVDYGEDYRGLQKLVSKGNQSAGYVVKKYNPESWLDGHLADSFCQVGGIYVNCMTDRVPNDMFIANGIEQWMRSPKMRQQDPRPESYHVLATHHRPSDKAFLTDVFAFDSTTGVLIEVILGISYVKIPKASMSKLLSRLTVNDSASCPTNMPLLSKSASVNLFDAPENLSTPSLSVAPTQQSAPALSLSKVKKVKNDGPDKGQLTQRIKSILAELSGLEIAEIKDDSELADLGIDSLMGMEMAHEIEKAFTISLPESDLMEVVDVPSLIKCVRKAMSGDADSAEYTTEQSTSEAADSDDKSTNYTTPSTPGEEALDMDKSMREFLGKEGTELNLPFETVMKAFNETKNMTDDRIAEYQQTRYVESVLPMQSQMCVSLVLEAFDQLNMRIRTAPAGEKFTRISHPKEHTRLVDYLYKMLEDASLINIDGEVITRTAIQVPRPSKEIFDELVSQHPDQNAADKLTFYTGSHLAEVLKGETDGIKLIFGTQDGRELVSKLYRDWPLNRLFYRQMEDFLERLTSKLDISQGVIKILEMGAGTGGTTKWLVPLLAKLNIPVEYTFTDIAPSFVAAARKKFSKQYPFMKFRTHDIEKAPADDLIGSQHVIIASNAVHATHSLSESGKNIRKALRPDGVLLMLEMTGTLHWVDIIFGLFEGWWYFDDGRTHAVTHESRWAKDLQAVGYGHVDWTDGVRPENKLEKLIIAFASGGRYERLHIPRPLESASADCAARQAVVDRYVQEMTAGFGAATGVSPSAPLAHQEPKGCCVLVTGATGSLGCHLLAALTSLPTIASVVCLNRRSRQDPLERQHRSLLEKKIFLSEETAARVRVIETDMSKPQLGLLEEEYNYLLNSVTHIVHNAWLMNAKLPLRRFEPQLQIMRNLLDLAYGISLQRPMEKVSFQFISSIATVGHWPIWTGKSSVPEERMAIESVLPTGYGDAKYICERMIDETLHKYPDRFRAMVVRPGQVAGSSTSGYWNTMEHFSFLVKSSQTLNALPDFDGVLSWTPVDVVASTLVDLLLLPEDKTPYSIYHIDNPVRQPWKEMNVVLADALHIPRSNIIPFEKWIQRVKDYPRQVEGAEGDNPAILLVDFLDNNFIRMSCGGLLLETKKSREHSKTLANLGPVSAETARLFIKSWIDMGFLSP

Sequence caution

The sequence CBF73494.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence EAA59557.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1745-1773Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AACD01000135
EMBL· GenBank· DDBJ
EAA59557.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
BN001302
EMBL· GenBank· DDBJ
CBF73494.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.

Genome annotation databases

Similar Proteins

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