Q5APD4 · C9MT_CANAL
- ProteinSphingolipid C9-methyltransferase
- GeneMTS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids513 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes methylation of the sphingoid base component of glucosylceramides (GluCers) at the C9-position. Sphingolipid C9-methylation requires 4,8-desaturated ceramides as substrates. Glucosylceramides play important roles in growth, differentiation and pathogenicity. The methyl group at the C9-position distinguishes fungal glucosylceramides from those of plants and animals, and may thus play a role in host-pathogen interactions enabling the host to recognize the fungal attack and initiate specific defense responses. Not necessary for vegetative growth at low temperatures, but plays a role in hyphal formation on solid medium.
Catalytic activity
- a (4E,8E)-4-sphinga-4,8-dienine ceramide + S-adenosyl-L-methionine = a 9-methyl-(4E,8E)-sphinga-4,8-dienine ceramide + H+ + S-adenosyl-L-homocysteine
Pathway
Lipid metabolism; sphingolipid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 222-223 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: YT | ||||||
Binding site | 259-267 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: VLDIGCGWG | ||||||
Binding site | 285-290 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TLGRNQ | ||||||
Binding site | 315-316 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: YR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular vesicle | |
Cellular Component | plasma membrane | |
Molecular Function | methyltransferase activity | |
Biological Process | filamentous growth | |
Biological Process | glucosylceramide biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSphingolipid C9-methyltransferase
- EC number
- Short namesC-9-MT
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Candida/Lodderomyces clade > Candida
Accessions
- Primary accessionQ5APD4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 52-72 | Helical | ||||
Sequence: ILFSILALVPGYITYKLGLGF | ||||||
Transmembrane | 74-94 | Helical | ||||
Sequence: TWVFFFLILAIPILMAYWSIM |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Produces only non-methylated glucosylceramides. Has a decreased hyphal growth rate.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434799 | 1-513 | Sphingolipid C9-methyltransferase | |||
Sequence: MLDDVAFIKTPAAKKQPPASNECGVWTTDSPAIHNAPLPADGPGSTSFSNTILFSILALVPGYITYKLGLGFKTWVFFFLILAIPILMAYWSIMSTFSPRINEKVKYPNRPISYYLEFHTPELKAKYETSNGGKGSKIPIETFQELYFDGKVSFKGDCLDVLEYKHDWASFRFTLGLFRFFLLGMIPEVIFHSQSQDEEQVRDHYDRGDDFYTWFLGPRMIYTSGVISDITREETLEELQDNKLTVMADKIDLKKGDHVLDIGCGWGTWTTFASSKYGANVTGITLGRNQTKWGNTLLKEYGIPSDQSRIVCCDYRDAPKSSKPSGKYDKITSVEMAEHVGIRRLTAYLEQCRDALEDDGLLFLQYSGLRKNWQYEDLEWGLFMNKYIFPGADASTPLSFFASCMESVGFEIVSVDNIGVHYSATLWRWYRNWIGNKDKVVNKYGVKWYRIWEFFLGSSVVASRNGTATCYQFICRKNINSYRRIDYVPQQKGLQGPVQEGTKWAKEFTNFYD |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length513
- Mass (Da)58,777
- Last updated2005-04-26 v1
- Checksum3CA020873BCD76B4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP017623 EMBL· GenBank· DDBJ | AOW26600.1 EMBL· GenBank· DDBJ | Genomic DNA |