Q5AG77 · GAP1_CANAL
- ProteinAmino-acid permease GAP1
- GeneGAP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids582 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Amino-acid permease that coordinates external nitrogen source response and morphogenesis (PubMed:12949183, PubMed:21764911, PubMed:28028545).
Is capable of transporting several structurally unrelated amino acids such as leucine and phenylalanine, though with a lower capacity than the GAP2 and GAP6 permeases (PubMed:21764911).
Has citrulline import activity (PubMed:12949183).
GAP1 is also able to transport thialysine, and thus probably also lysine (PubMed:21764911).
Functions as a sensor via detection of some amino acids including methionine, leading to a rapid activation of trehalase, a downstream target of PKA (PubMed:21764911).
Is capable of transporting several structurally unrelated amino acids such as leucine and phenylalanine, though with a lower capacity than the GAP2 and GAP6 permeases (PubMed:21764911).
Has citrulline import activity (PubMed:12949183).
GAP1 is also able to transport thialysine, and thus probably also lysine (PubMed:21764911).
Functions as a sensor via detection of some amino acids including methionine, leading to a rapid activation of trehalase, a downstream target of PKA (PubMed:21764911).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell surface | |
Cellular Component | fungal-type cell wall | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | amino acid transmembrane transporter activity | |
Biological Process | amino acid transmembrane transport | |
Biological Process | amino acid transport | |
Biological Process | filamentous growth | |
Biological Process | filamentous growth of a population of unicellular organisms |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAmino-acid permease GAP1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Candida/Lodderomyces clade > Candida
Accessions
- Primary accessionQ5AG77
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 82-102 | Helical | ||||
Sequence: MIAIGSSIGTGLFVGTGGALS | ||||||
Transmembrane | 120-142 | Helical | ||||
Sequence: VFMTMQGLGELAVAFPVSGGFNL | ||||||
Transmembrane | 150-170 | Helical | ||||
Sequence: PGIGFAVGWNYFLQFFVLLPL | ||||||
Transmembrane | 190-210 | Helical | ||||
Sequence: VFVIIFWFVVLVITMLGVRWY | ||||||
Transmembrane | 216-236 | Helical | ||||
Sequence: VFCTIKVIAVIGFIILGIVLI | ||||||
Transmembrane | 263-283 | Helical | ||||
Sequence: GFASSLITAAFSFGGTEMIAL | ||||||
Transmembrane | 302-322 | Helical | ||||
Sequence: VFWRIVIFYLGSIIMIATLVP | ||||||
Transmembrane | 343-363 | Helical | ||||
Sequence: IAIVNGGIKGLPSVINAVILI | ||||||
Transmembrane | 402-422 | Helical | ||||
Sequence: PLFAILITNVFGLFALIAADN | ||||||
Transmembrane | 427-447 | Helical | ||||
Sequence: VAFNWLLALSGLSSIFTWMSI | ||||||
Transmembrane | 481-501 | Helical | ||||
Sequence: FGLTLNILYLIAQFYIGLFPV | ||||||
Transmembrane | 510-530 | Helical | ||||
Sequence: FFLAYLGVPVILASWIGYKIW |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000439806 | 1-582 | Amino-acid permease GAP1 | |||
Sequence: MLHKKETNDTFVQLNRSPSTGEQKSSGIWSSIKDSFKPALPQDKLTGVDDIPDRELTDIERININAANSNLQRKLKTRHLQMIAIGSSIGTGLFVGTGGALSTGGPAAIVLAWAISAISVFMTMQGLGELAVAFPVSGGFNLYASKFLEPGIGFAVGWNYFLQFFVLLPLELVAGAITIKYWNASINSDVFVIIFWFVVLVITMLGVRWYGEAELVFCTIKVIAVIGFIILGIVLICGGGPNHEFIGGKYWREPGPFANSFKGFASSLITAAFSFGGTEMIALTASESSNVRHALPKAIKQVFWRIVIFYLGSIIMIATLVPYNDKRLLGSSSVDVTASPFTIAIVNGGIKGLPSVINAVILISVLSVGNASVYATSRTLNSLAEQGMAPKWTGYIDRAGRPLFAILITNVFGLFALIAADNEKQVVAFNWLLALSGLSSIFTWMSINLSHIRFRRAMKVQNRSLTELPFVAQSGVWGSYFGLTLNILYLIAQFYIGLFPVGGKPNAYDFFLAYLGVPVILASWIGYKIWKRDWTLFIRAKDIDLDTGRINVDLDLLQQEIAEEKAQLAEKPFYIRIYRFWC | ||||||
Glycosylation | 8 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 183 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 370 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 448 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 462 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Induction
Expression is under control of the CSY1 amino-acid sensor (PubMed:28028545).
Expression is induced by N-acetylglucosamine (PubMed:12949183).
Expression is also regulated by CPH1-mediated RAS1 signaling but is independent of EFG1 (PubMed:12949183).
Induced during biofilm development (PubMed:21414038, PubMed:22265407, PubMed:23572557).
Induced upon internalization by host macrophages (PubMed:15470236).
Expression is down-regulated by growth in alkaline conditions (PubMed:15554973).
Expression is also controlled by the nitrogen-dependent GATA-type transcriptional activator GAT1 (PubMed:14617156).
Expression is induced by N-acetylglucosamine (PubMed:12949183).
Expression is also regulated by CPH1-mediated RAS1 signaling but is independent of EFG1 (PubMed:12949183).
Induced during biofilm development (PubMed:21414038, PubMed:22265407, PubMed:23572557).
Induced upon internalization by host macrophages (PubMed:15470236).
Expression is down-regulated by growth in alkaline conditions (PubMed:15554973).
Expression is also controlled by the nitrogen-dependent GATA-type transcriptional activator GAT1 (PubMed:14617156).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-28 | Disordered | ||||
Sequence: MLHKKETNDTFVQLNRSPSTGEQKSSGI | ||||||
Compositional bias | 11-28 | Polar residues | ||||
Sequence: FVQLNRSPSTGEQKSSGI |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length582
- Mass (Da)63,951
- Last updated2005-04-26 v1
- Checksum72CC98F97D0E4053
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 11-28 | Polar residues | ||||
Sequence: FVQLNRSPSTGEQKSSGI |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP017627 EMBL· GenBank· DDBJ | AOW29704.1 EMBL· GenBank· DDBJ | Genomic DNA |