Q5A309 · DOT1_CANAL

Function

function

Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Catalytic activity

Activity regulation

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site1110-1113S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site1133-1142S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site1160S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site1197-1198S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular Functionhistone H3K79 methyltransferase activity
Molecular Functionhistone H3K79 trimethyltransferase activity
Biological ProcessDNA damage checkpoint signaling
Biological ProcessDNA repair
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
  • EC number
  • Alternative names
    • Histone H3-K79 methyltransferase (H3-K79-HMTase)

Gene names

    • Name
      DOT1
    • ORF names
      CaO19.7402
    • Ordered locus names
      CAALFM_C306300WA

Organism names

Accessions

  • Primary accession
    Q5A309
  • Secondary accessions
    • A0A1D8PKG3

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002706061-1343Histone-lysine N-methyltransferase, H3 lysine-79 specific

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-29Disordered
Region210-236Disordered
Region418-439Disordered
Domain987-1304DOT1

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,343
  • Mass (Da)
    155,678
  • Last updated
    2005-04-26 v1
  • Checksum
    D4DE4546A3DD562C
MISGHLQTPDSSDHSGDEAKLTKPSGLESKTNELWSSDLEEELESRIMQPATFSSILKQFPSISEETIISKIMSNKNCDKNNWKKKIYCYRYFLQPSKEEPDVGNMKSLVEKLEKLKLKKWSASEIEQLFCNYLEDLTTSAVKVSIPGKTLDEVAAAVNNIHPRPRWTRKEIECLIKNENDFKKLEKDLFVRDLDSIKKKIRRDNLSIQNEIQDSEKKSPQSTDSNNKDSSRDLSRKERDQLKKLLSKPICFSELLAKFPGHSWEYIAREIIQLDSSEDNTIWLKKIYYYCVVYSISVDKEITKYIGGGNRIYEKVRSDWSKIKTLDFFKDWSLQEFERLYCFAFHDLTKSALTKNFPSKNLNDICKVVNISFPKVPYTDREMKYLERHLDTPMQTLENNLPFRSRGSIHKKLEALKALTETTEQKQPPTKTRPKNEEEKNVENAAYMKELIMFDLTLEAIENTFPSEPIEEIIKEIKNSEIFDPLSFTRGEKELMAKLVKKGNLIDDCFDYFPLREEEFIRSKYAEAEYVSGRKMKFNTPEERLAYEAKWTLFNMGKQEYGRGNRRSTKRYCEIDELSKLEQEASVKRSKKKIELTEEELEQRRKRSEHFRLCRLKKLEEKREKYRIEKAKRLEKIAAGLIKPSTSGYELKDIVTSAEYFQSIVGDKQKVQEGQKRKRIQTEYFAPEFIEKPKAVKLKTTKRQAEKNKIKKQLKREAQLKIKKKKTIAPKKGKRRVKTNNGIIEEIKDVYKLSSEPYVESEVEEEDEEEDYISPYDPPDIISDSQVKLNGRHLYISSFYKELPEIPELKFVSLPHMEMSGNDITVAKQIMTTANDDILYDDCLAYEIVAQHIKSYRDLPISFPPVLDPITHELNSANIVRIRFFLYPEHYESFMLASPKSNELDPVHEIAKLFMIQYSLYFSHSDTLKKIITEDYCHKLEHSVEENDFGEFMFVVDKWNQLVMKLSPNLASVQNILGLKEDINEAPRAYLNQQEVSIPTNSDLKIETFYDEIMYESASPLFNPINSNLEIDSESAPIPLGEVEIPNNVIEEINEKMPDNYIPDFFRRLKEKTEVSRYAMQQILLRVYSRVVSTDSRKLRSYKAFTAETYGELLPSFTSEVLEKLNLLPTQKFYDLGSGVGNTTFQAALEFGACSSGGCEIMEHASKLTELQAGLIQKHLAVLGLQKLNLDFALHESFVGNEKVRASCLDCDVLIINNYLFDGQLNDEVGKLLYGLRPGTKIVSLRNFISPRYRATFDTVFDFLSVEKHEMSDIMSVSWTANKVPYYISTVEETIPREYLSREETKETSGKSKSVSPVGEIENVAAAMMTPPTDSSESEIIKN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP017625
EMBL· GenBank· DDBJ
AOW28637.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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