Q5A309 · DOT1_CANAL
- ProteinHistone-lysine N-methyltransferase, H3 lysine-79 specific
- GeneDOT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1343 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.
Miscellaneous
In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Catalytic activity
- L-lysyl79-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl79-[histone H3] + 3 S-adenosyl-L-homocysteine
RHEA-COMP:15549 CHEBI:29969 Position: 79+ 3 CHEBI:59789 = 3 CHEBI:15378 + RHEA-COMP:15552 CHEBI:61961 Position: 79+ 3 CHEBI:57856
Activity regulation
Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 1110-1113 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: YGEL | ||||||
Binding site | 1133-1142 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: FYDLGSGVGN | ||||||
Binding site | 1160 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 1197-1198 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: SF |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | histone H3K79 methyltransferase activity | |
Molecular Function | histone H3K79 trimethyltransferase activity | |
Biological Process | DNA damage checkpoint signaling | |
Biological Process | DNA repair | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone-lysine N-methyltransferase, H3 lysine-79 specific
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Candida/Lodderomyces clade > Candida
Accessions
- Primary accessionQ5A309
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000270606 | 1-1343 | Histone-lysine N-methyltransferase, H3 lysine-79 specific | |||
Sequence: MISGHLQTPDSSDHSGDEAKLTKPSGLESKTNELWSSDLEEELESRIMQPATFSSILKQFPSISEETIISKIMSNKNCDKNNWKKKIYCYRYFLQPSKEEPDVGNMKSLVEKLEKLKLKKWSASEIEQLFCNYLEDLTTSAVKVSIPGKTLDEVAAAVNNIHPRPRWTRKEIECLIKNENDFKKLEKDLFVRDLDSIKKKIRRDNLSIQNEIQDSEKKSPQSTDSNNKDSSRDLSRKERDQLKKLLSKPICFSELLAKFPGHSWEYIAREIIQLDSSEDNTIWLKKIYYYCVVYSISVDKEITKYIGGGNRIYEKVRSDWSKIKTLDFFKDWSLQEFERLYCFAFHDLTKSALTKNFPSKNLNDICKVVNISFPKVPYTDREMKYLERHLDTPMQTLENNLPFRSRGSIHKKLEALKALTETTEQKQPPTKTRPKNEEEKNVENAAYMKELIMFDLTLEAIENTFPSEPIEEIIKEIKNSEIFDPLSFTRGEKELMAKLVKKGNLIDDCFDYFPLREEEFIRSKYAEAEYVSGRKMKFNTPEERLAYEAKWTLFNMGKQEYGRGNRRSTKRYCEIDELSKLEQEASVKRSKKKIELTEEELEQRRKRSEHFRLCRLKKLEEKREKYRIEKAKRLEKIAAGLIKPSTSGYELKDIVTSAEYFQSIVGDKQKVQEGQKRKRIQTEYFAPEFIEKPKAVKLKTTKRQAEKNKIKKQLKREAQLKIKKKKTIAPKKGKRRVKTNNGIIEEIKDVYKLSSEPYVESEVEEEDEEEDYISPYDPPDIISDSQVKLNGRHLYISSFYKELPEIPELKFVSLPHMEMSGNDITVAKQIMTTANDDILYDDCLAYEIVAQHIKSYRDLPISFPPVLDPITHELNSANIVRIRFFLYPEHYESFMLASPKSNELDPVHEIAKLFMIQYSLYFSHSDTLKKIITEDYCHKLEHSVEENDFGEFMFVVDKWNQLVMKLSPNLASVQNILGLKEDINEAPRAYLNQQEVSIPTNSDLKIETFYDEIMYESASPLFNPINSNLEIDSESAPIPLGEVEIPNNVIEEINEKMPDNYIPDFFRRLKEKTEVSRYAMQQILLRVYSRVVSTDSRKLRSYKAFTAETYGELLPSFTSEVLEKLNLLPTQKFYDLGSGVGNTTFQAALEFGACSSGGCEIMEHASKLTELQAGLIQKHLAVLGLQKLNLDFALHESFVGNEKVRASCLDCDVLIINNYLFDGQLNDEVGKLLYGLRPGTKIVSLRNFISPRYRATFDTVFDFLSVEKHEMSDIMSVSWTANKVPYYISTVEETIPREYLSREETKETSGKSKSVSPVGEIENVAAAMMTPPTDSSESEIIKN |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-29 | Disordered | ||||
Sequence: MISGHLQTPDSSDHSGDEAKLTKPSGLES | ||||||
Region | 210-236 | Disordered | ||||
Sequence: NEIQDSEKKSPQSTDSNNKDSSRDLSR | ||||||
Region | 418-439 | Disordered | ||||
Sequence: ALTETTEQKQPPTKTRPKNEEE | ||||||
Domain | 987-1304 | DOT1 | ||||
Sequence: PRAYLNQQEVSIPTNSDLKIETFYDEIMYESASPLFNPINSNLEIDSESAPIPLGEVEIPNNVIEEINEKMPDNYIPDFFRRLKEKTEVSRYAMQQILLRVYSRVVSTDSRKLRSYKAFTAETYGELLPSFTSEVLEKLNLLPTQKFYDLGSGVGNTTFQAALEFGACSSGGCEIMEHASKLTELQAGLIQKHLAVLGLQKLNLDFALHESFVGNEKVRASCLDCDVLIINNYLFDGQLNDEVGKLLYGLRPGTKIVSLRNFISPRYRATFDTVFDFLSVEKHEMSDIMSVSWTANKVPYYISTVEETIPREYLSREE |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,343
- Mass (Da)155,678
- Last updated2005-04-26 v1
- ChecksumD4DE4546A3DD562C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP017625 EMBL· GenBank· DDBJ | AOW28637.1 EMBL· GenBank· DDBJ | Genomic DNA |