Q59675 · XY10C_CELJA

Function

function

Endo-acting xylanase which specifically cleaves internal linkages on the xylan backbone, releasing xylooligosaccharides. Is able to hydrolyze oat spelt xylan, the arabinoxylans from wheat and rye, and glucuronoxylan. Also displays very low activity against xylooligosaccharides. During the xylan degradation process, Xyn10C may act on the soluble xylans and long xylooligosaccharides products released by the secreted xylanases Xyn11A, Xyn11B and Xyn10A.

Catalytic activity

  • Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
    EC:3.2.1.8 (UniProtKB | ENZYME | Rhea)

Pathway

Glycan degradation; xylan degradation.

Features

Showing features for binding site, active site.

160650100150200250300350400450500550600
TypeIDPosition(s)Description
Binding site106a carbohydrate (UniProtKB | ChEBI)
Binding site171a carbohydrate (UniProtKB | ChEBI)
Binding site217a carbohydrate (UniProtKB | ChEBI)
Binding site296-299substrate
Binding site332substrate
Binding site384substrate
Active site385Proton donor
Active site497Nucleophile
Binding site552substrate

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell outer membrane
Molecular Functionendo-1,4-beta-xylanase activity
Biological Processxylan catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM15Carbohydrate-Binding Module Family 15
    • GH10Glycoside Hydrolase Family 10

Names & Taxonomy

Protein names

  • Recommended name
    Endo-beta-1,4-xylanase Xyn10C
  • EC number
  • Short names
    Xylanase 10C
  • Alternative names
    • XYLF

Gene names

    • Name
      xyn10C
    • Synonyms
      xynF

Organism names

Accessions

  • Primary accession
    Q59675

Subcellular Location

Cell outer membrane
; Lipid-anchor
Note: Is predominantly associated with the cell membrane.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis340Significantly reduces the catalytic activity against xylotetraose and xylan. Also modifies the cleavage pattern of xylotetraose.

Chemistry

PTM/Processing

Features

Showing features for signal, lipidation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-19
Lipidation20N-palmitoyl cysteine
Lipidation20S-diacylglycerol cysteine
ChainPRO_500014760920-606Endo-beta-1,4-xylanase Xyn10C
Disulfide bond183↔200

Keywords

Expression

Induction

Induced when the bacterium is cultured on xylan or beta-glucan but not on medium containing mannan. Is repressed by glucose.

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region23-64Disordered
Compositional bias26-64Polar residues
Domain91-242CBM15
Domain245-596GH10

Domain

The N-terminal CBM15 domain binds xylan, including decorated xylans and xylooligosaccharides, but the physiological role of this domain is unclear. It may act as a product capture system: large xylooligosaccharides generated by Xyn10C would bind to CBM15 and this would restrict the diffusion of these polymers into the environment and therefore increase the selective utilization of these molecules by C.japonicus. Xylanase activity resides in the C-terminal domain.

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    606
  • Mass (Da)
    64,884
  • Last updated
    2004-03-01 v2
  • Checksum
    56E149954CE6BFEE
MKKIQQLLMLSLISSTLIACGGGGGGGSTPTTSSSPQSSSPASTPSSASSSSIISSSSLSSSLSSSSLSSSSLSSSSASSVSSSSVAASEGNVVIEVDMANGWRGNASGSTSHSGITYSADGVTFAALGDGVGAVFDIARPTTLEDAVIAMVVNVSAEFKASEANLQIFAQLKEDWSKGEWDCLAASSELTADTDLTLTCTIDEDDDKFNQTARDVQVGIQAKGTPAGTITIKSVTITLAQEAYSANVDHLRDLAPSDFPIGVAVSNTDSATYNLLTNSREQAVVKKHFNHLTAGNIMKMSYMQPTEGNFNFTNADAFVDWATENNMTVHGHALVWHSDYQVPNFMKNWAGSAEDFLAALDTHITTIVDHYEAKGNLVSWDVVNEAIDDNSPANFRTTDSAFYVKSGNSSVYIERAFQTARAADPAVILYYNDYNIEQNNAKTTKMVDMVKDFQARSIPIDGVGFQMHVCMNYPSIANISAAMKKVVDLGLLVKITELDVAVNQPHCDAYPANKINPLTEAAQLAQKKRYCDVVKAYLDTVPVNQRGGISVWGTTDANTWLDGLYREQFEDEKISWPLLFDNNYNDKPALRGFADALIGTQCTNTH

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias26-64Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z48928
EMBL· GenBank· DDBJ
CAA88764.2
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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