Q59458 · RBL1A_HYDMR

  • Protein
    Ribulose bisphosphate carboxylase large chain 1
  • Gene
    cbbL1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

Vmax pH TEMPERATURE[C] NOTES EVIDENCE
1.2 μmol/min/mgwith CO2 as substrate, expressed in E.coli
The CO2/O2 specificity factor (tau) is 26.6.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site115substrate; in homodimeric partner
Binding site165substrate
Active site167Proton acceptor
Binding site169substrate
Binding site193Mg2+ (UniProtKB | ChEBI); via carbamate group
Binding site195Mg2+ (UniProtKB | ChEBI)
Binding site196Mg2+ (UniProtKB | ChEBI)
Active site286Proton acceptor
Binding site287substrate
Binding site319substrate
Site326Transition state stabilizer
Binding site371substrate

GO annotations

AspectTerm
Molecular Functionmagnesium ion binding
Molecular Functionmonooxygenase activity
Molecular Functionribulose-bisphosphate carboxylase activity
Biological Processreductive pentose-phosphate cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribulose bisphosphate carboxylase large chain 1
  • EC number
  • Short names
    RuBisCO large subunit 1

Gene names

    • Name
      cbbL1
    • Synonyms
      cbbL-1

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 11271 / JCM 7688 / MH-110
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Piscirickettsiaceae > Hydrogenovibrio

Accessions

  • Primary accession
    Q59458
  • Secondary accessions
    • Q75W45

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000626241-472Ribulose bisphosphate carboxylase large chain 1
Modified residue193N6-carboxylysine

Expression

Induction

Both mRNA and protein accumulate at 2% and 0.03% CO2, but not at 15% or 0.15% CO2 (at protein level).

Interaction

Subunit

Heterohexadecamer of 8 large chains and 8 small chains.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    472
  • Mass (Da)
    52,488
  • Last updated
    1996-11-01 v1
  • Checksum
    71FCAE86F7CF2530
MAKTYNAGVKEYRETYWMPEYEPKDSDFLACFKVVPQPGVPREEIAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYRIEDVPGDDSAFYAFIAYPIDLFEEGSIVSVMTSLVGNVFGFKALRSIRLEDIRFPLAYVMTCGGPPHGIQVERDKMDKYGRPMLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVTSQPFMRWRDRFLFCQDAIEKAQDETGERTGHYLNATAGTPEEMYERAEFAKEIGSPIVMHDFLTGGLTANTGLANYCRKNGLLLHIHRAMHGVIDRNPLHGIHFRVLSKVLRLSGGDHLHSGTVVGKLEGDRGSDLGWIDIMRDSFIAEDRSRGIMFDQDFGEMPGVIPVASGGIHVWHMPALVAIFGDDSVLQFGGGTIGHPWGNAVGAAVNLVALEACVQARNEGQEIEKNGKEILTNDGKHSPELKIAMETWKEIKFEFDTVDKLDLSHK

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict298in Ref. 2; BAD15307
Sequence conflict332-334in Ref. 2; BAD15307
Sequence conflict399in Ref. 2; BAD15307
Sequence conflict407in Ref. 2; BAD15307
Sequence conflict411-413in Ref. 2; BAD15307
Sequence conflict440-441in Ref. 2; BAD15307

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D43621
EMBL· GenBank· DDBJ
BAA07729.1
EMBL· GenBank· DDBJ
Genomic DNA
AB122069
EMBL· GenBank· DDBJ
BAD15307.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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