Q59289 · HEPC_PEDHD

Function

function

Specifically cleaves heparan sulfate-rich regions of acidic polysaccharides. Does not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages. Functions in cleaving metazoan heparan sulfate and providing carbon, nitrogen and sulfate sources for microorganisms.

Catalytic activity

  • Elimination of sulfate, appears to act on linkages between N-acetyl-D-glucosamine and uronate. Product is an unsaturated sugar.
    EC:4.2.2.8 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
143 μMheparan sulfate
80 μMheparan sulfatewith recombinant enzyme
kcat is 94 sec-1. kcat is 78 sec-1 with recombinant enzyme.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site294Proton acceptor

GO annotations

AspectTerm
Cellular Componentperiplasmic space
Molecular Functionheparin-sulfate lyase activity
Biological Processheparan sulfate proteoglycan catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Heparin-sulfate lyase
  • EC number
  • Alternative names
    • Heparin-sulfate eliminase
    • Heparinase III (HepIII)
    • Heparitin-sulfate lyase

Gene names

    • Name
      hepC
    • Ordered locus names
      Phep_3797

Organism names

Accessions

  • Primary accession
    Q59289
  • Secondary accessions
    • C6XUY0

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis295Impaired catalytic activity.
Mutagenesis510Impaired catalytic activity.

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-24
ChainPRO_500014461425-659Heparin-sulfate lyase

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the polysaccharide lyase 12 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    659
  • Mass (Da)
    75,807
  • Last updated
    1996-11-01 v1
  • Checksum
    B73EDF10A1256FE2
MTTKIFKRIIVFAVIALSSGNILAQSSSITRKDFDHINLEYSGLEKVNKAVAAGNYDDAAKALLAYYREKSKAREPDFSNAEKPADIRQPIDKVTREMADKALVHQFQPHKGYGYFDYGKDINWQMWPVKDNEVRWQLHRVKWWQAMALVYHATGDEKYAREWVYQYSDWARKNPLGLSQDNDKFVWRPLEVSDRVQSLPPTFSLFVNSPAFTPAFLMEFLNSYHQQADYLSTHYAEQGNHRLFEAQRNLFAGVSFPEFKDSPRWRQTGISVLNTEIKKQVYADGMQFELSPIYHVAAIDIFLKAYGSAKRVNLEKEFPQSYVQTVENMIMALISISLPDYNTPMFGDSWITDKNFRMAQFASWARVFPANQAIKYFATDGKQGKAPNFLSKALSNAGFYTFRSGWDKNATVMVLKASPPGEFHAQPDNGTFELFIKGRNFTPDAGVFVYSGDEAIMKLRNWYRQTRIHSTLTLDNQNMVITKARQNKWETGNNLDVLTYTNPSYPNLDHQRSVLFINKKYFLVIDRAIGEATGNLGVHWQLKEDSNPVFDKTKNRVYTTYRDGNNLMIQSLNADRTSLNEEEGKVSYVYNKELKRPAFVFEKPKKNAGTQNFVSIVYPYDGQKAPEISIRENKGNDFEKGKLNLTLTINGKQQLVLVP

Sequence caution

The sequence ACU05988.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict128-129in Ref. 1; AA sequence

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U27586
EMBL· GenBank· DDBJ
AAB18278.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001681
EMBL· GenBank· DDBJ
ACU05988.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

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