Q59212 · TYSY_BACLI

  • Protein
    Thymidylate synthase
  • Gene
    thyA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

127920406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site141-142dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site161Nucleophile
Binding site181-184dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site184(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site192dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site222-224dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site278(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • Synonyms
      thyBL

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 9789 / DSM 8785 / NBRC 12195 / NCIMB 6346 / NCTC 6346 / IMET 11025 / NRS 243
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    Q59212

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001409271-279Thymidylate synthase

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    279
  • Mass (Da)
    32,760
  • Last updated
    1996-11-01 v1
  • Checksum
    479D3E24E452EA8F
MSHYDQQYNAIIQKIIESGISDEEYQVRTKWDSDGTPAHTLSIMSEKMRFDNSEVPILTTKKVAWKTAIKELLWIWQLKSNDVQVLNDMGVHIWDQWRLEDGTIGAAYGYQLGKKNRTVNGQKVDQVDYLLHQLKHNPSSRRHLTMLWNPDDLDGMALTPCVYETQWYVKEGKLSLEVRARSNDMALGNPFNVFQYNVLQRMIAQVLGYELGEYIFNIGDCHIYTRHIDNLNIQMKREQYEAPKLWINPDIKNFYDFTIDDFKLIDYKHGDKLTFEVAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z50165
EMBL· GenBank· DDBJ
CAA90533.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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