Q58085 · RIB7_METJA

Function

function

Catalyzes an early step in riboflavin biosynthesis, the NAD(P)H-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate. The beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerization. NADPH and NADH function equally well as the reductants. Does not catalyze the reduction of 5-amino-6-(5-phospho-D-ribosylamino)uracil to 5-amino-6-(5-phospho-D-ribitylamino)uracil.

Miscellaneous

The protein sequence in PubMed:16730025 comes from protein expressed and processed in E.coli.
Using chirally deuterated NADPH, the enzyme was shown to be A-type reductase catalyzing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate.

Catalytic activity

Pathway

Cofactor biosynthesis; riboflavin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site16NADP+ (UniProtKB | ChEBI)
Binding site57NADP+ (UniProtKB | ChEBI)
Binding site61NADP+ (UniProtKB | ChEBI)
Binding site83-86NADP+ (UniProtKB | ChEBI)
Binding site134NADP+ (UniProtKB | ChEBI)
Binding site156-159NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Molecular Functionoxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Molecular Functionprotein dimerization activity
Biological Processriboflavin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase
  • EC number
  • Short names
    DAROPP reductase; DARP reductase
  • Alternative names
    • 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase
    • 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase (DARIPP synthase)
    • MjaRED

Gene names

    • Name
      arfC
    • Ordered locus names
      MJ0671

Organism names

Accessions

  • Primary accession
    Q58085

Proteomes

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001359442-2242,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the HTP reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    224
  • Mass (Da)
    25,037
  • Last updated
    1996-11-01 v1
  • Checksum
    4D8C15CE291E89D8
MVMVMEKKPYIISNVGMTLDGKLATINNDSRISCEEDLIRVHKIRANVDGIMVGIGTVLKDDPRLTVHKIKSDRNPVRIVVDSKLRVPLNARVLNKDAKTIIATTEDTNEEKEKKIKILEDMGVEVVKCGRGKVDLKKLMDILYDKGIKSILLEGGGTLNWGMFKEGLVDEVSVYIAPKIFGGKEAPTYVDGEGFKTVDECVKLELKNFYRLGEGIVLEFKVKK

Mass Spectrometry

Molecular mass is 24,906 Da. Determined by Electrospray.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L77117
EMBL· GenBank· DDBJ
AAB98665.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp