Q57849 · NK_METJA
- ProteinNucleoside kinase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids302 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the phosphorylation of a wide range of nucleosides to yield nucleoside monophosphates. Shows the highest activity for inosine, guanosine and cytidine, but very poor kinase activity with adenosine, thymidine, uridine and xanthosine. ATP is the best phosphate donor, but can also use ITP and GTP. Shows extremely low activity with fructose-6-phosphate.
Catalytic activity
- cytidine + ATP = CMP + ADP + H+
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Can use Mg2+ and Mn2+ with equal efficiency in vitro, and to a lesser extent, Ni2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.25 mM | ATP | 70 | ||||
17 μM | cytidine | 50 | ||||
20 μM | cytidine | 70 | ||||
20 μM | inosine | 70 | ||||
21 μM | inosine | 50 | ||||
62 μM | guanosine | 70 | ||||
78 μM | guanosine | 50 | ||||
160 μM | adenosine | 70 | ||||
180 μM | uridine | 50 | ||||
200 μM | uridine | 70 | ||||
230 μM | adenosine | 50 | ||||
300 μM | ribose | 70 | ||||
710 μM | xanthosine | 50 | ||||
900 μM | thymidine | 50 | ||||
1000 μM | thymidine | 70 | ||||
1800 μM | fructose | 70 | ||||
2200 μM | 2-deoxy-adenosine | 70 | ||||
2500 μM | 2-deoxy-adenosine | 50 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
120 μmol/min/mg | 70 | with guanosine as substrate | |||
75 μmol/min/mg | 70 | with inosine as substrate | |||
70 μmol/min/mg | 70 | with cytidine as substrate | |||
29.3 μmol/min/mg | 50 | with guanosine as substrate | |||
18.8 μmol/min/mg | 50 | with inosine as substrate | |||
9 μmol/min/mg | 50 | with cytidine as substrate | |||
4 μmol/min/mg | 70 | with adenosine as substrate | |||
1 μmol/min/mg | 50 | with adenosine as substrate | |||
0.25 μmol/min/mg | 70 | with uridine as substrate | |||
0.6 μmol/min/mg | 50 | with xanthosine as substrate | |||
0.6 μmol/min/mg | 70 | with ribose as substrate | |||
0.2 μmol/min/mg | 70 | with 2-deoxy-adenosine as substrate | |||
0.08 μmol/min/mg | 70 | with thymidine as substrate | |||
0.05 μmol/min/mg | 50 | with 2-deoxy-adenosine as substrate | |||
0.04 μmol/min/mg | 50 | with uridine as substrate | |||
0.03 μmol/min/mg | 70 | with fructose as substrate | |||
0.01 μmol/min/mg | 50 | with thymidine as substrate |
pH Dependence
Optimum pH is 7.0. 50% of activity is found at pH 6.1 and 8.2.
Temperature Dependence
Optimum temperature is 85 degrees Celsius. Thermostable.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17 | substrate | ||||
Sequence: D | ||||||
Binding site | 33 | substrate | ||||
Sequence: Q | ||||||
Binding site | 43 | substrate | ||||
Sequence: G | ||||||
Binding site | 47 | substrate | ||||
Sequence: N | ||||||
Binding site | 109 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 111-113 | substrate | ||||
Sequence: TFF | ||||||
Binding site | 163 | substrate | ||||
Sequence: Q | ||||||
Binding site | 186 | ATP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 214-219 | ATP (UniProtKB | ChEBI) | ||||
Sequence: TKGSKG | ||||||
Active site | 247 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 247 | substrate | ||||
Sequence: D | ||||||
Site | 250 | Transition state stabilizer | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | cytidine kinase activity | |
Molecular Function | guanosine kinase activity | |
Molecular Function | inosine kinase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | nucleoside kinase activity | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleoside kinase
- Short namesNK
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanococci > Methanococcales > Methanocaldococcaceae > Methanocaldococcus
Accessions
- Primary accessionQ57849
Proteomes
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000080152 | 2-302 | Nucleoside kinase | |||
Sequence: GGKMEKITCVGHTALDYIFNVEKFPEPNTSIQIPSARKYYGGAAANTAVGIKKLGVNSELLSCVGYDFKNSGYERYLKNLDINISKLYYSEEEETPKAWIFTDKDNNQITFFLWGAAKHYKELNPPNFNTEIVHIATGDPEFNLKCAKKAYGNNLVSFDPGQDLPQYSKEMLLEIIEHTNFLFMNKHEFERASNLLNFEIDDYLERVDALIVTKGSKGSVIYTKDKKIEIPCIKAGKVIDPTGAGDSYRAGFLSAYVKGYDLEKCGLIGAATASFVVEAKGCQTNLPTWDKVVERLEKHRI |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length302
- Mass (Da)33,920
- Last updated1996-11-01 v1
- Checksum6A535606CC7D3260
Mass Spectrometry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L77117 EMBL· GenBank· DDBJ | AAB98396.1 EMBL· GenBank· DDBJ | Genomic DNA |