Q57849 · NK_METJA

Function

function

Catalyzes the phosphorylation of a wide range of nucleosides to yield nucleoside monophosphates. Shows the highest activity for inosine, guanosine and cytidine, but very poor kinase activity with adenosine, thymidine, uridine and xanthosine. ATP is the best phosphate donor, but can also use ITP and GTP. Shows extremely low activity with fructose-6-phosphate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Can use Mg2+ and Mn2+ with equal efficiency in vitro, and to a lesser extent, Ni2+.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.25 mMATP70
17 μMcytidine50
20 μMcytidine70
20 μMinosine70
21 μMinosine50
62 μMguanosine70
78 μMguanosine50
160 μMadenosine70
180 μMuridine50
200 μMuridine70
230 μMadenosine50
300 μMribose70
710 μMxanthosine50
900 μMthymidine50
1000 μMthymidine70
1800 μMfructose70
2200 μM2-deoxy-adenosine70
2500 μM2-deoxy-adenosine50
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
120 μmol/min/mg70with guanosine as substrate
75 μmol/min/mg70with inosine as substrate
70 μmol/min/mg70with cytidine as substrate
29.3 μmol/min/mg50with guanosine as substrate
18.8 μmol/min/mg50with inosine as substrate
9 μmol/min/mg50with cytidine as substrate
4 μmol/min/mg70with adenosine as substrate
1 μmol/min/mg50with adenosine as substrate
0.25 μmol/min/mg70with uridine as substrate
0.6 μmol/min/mg50with xanthosine as substrate
0.6 μmol/min/mg70with ribose as substrate
0.2 μmol/min/mg70with 2-deoxy-adenosine as substrate
0.08 μmol/min/mg70with thymidine as substrate
0.05 μmol/min/mg50with 2-deoxy-adenosine as substrate
0.04 μmol/min/mg50with uridine as substrate
0.03 μmol/min/mg70with fructose as substrate
0.01 μmol/min/mg50with thymidine as substrate

pH Dependence

Optimum pH is 7.0. 50% of activity is found at pH 6.1 and 8.2.

Temperature Dependence

Optimum temperature is 85 degrees Celsius. Thermostable.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site17substrate
Binding site33substrate
Binding site43substrate
Binding site47substrate
Binding site109ATP (UniProtKB | ChEBI)
Binding site111-113substrate
Binding site163substrate
Binding site186ATP (UniProtKB | ChEBI)
Binding site214-219ATP (UniProtKB | ChEBI)
Active site247Proton acceptor
Binding site247substrate
Site250Transition state stabilizer

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncytidine kinase activity
Molecular Functionguanosine kinase activity
Molecular Functioninosine kinase activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleoside kinase activity
Biological Processphosphorylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoside kinase
  • Short names
    NK
  • Alternative names
    • ATP-dependent nucleoside monophosphokinase
    • Cytidine kinase
      (EC:2.7.1.213
      ) . EC:2.7.1.213 (UniProtKB | ENZYME | Rhea)
    • Guanosine-inosine kinase
      (EC:2.7.1.73
      ) . EC:2.7.1.73 (UniProtKB | ENZYME | Rhea)

Gene names

    • Ordered locus names
      MJ0406

Organism names

Accessions

  • Primary accession
    Q57849

Proteomes

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000801522-302Nucleoside kinase

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    302
  • Mass (Da)
    33,920
  • Last updated
    1996-11-01 v1
  • Checksum
    6A535606CC7D3260
MGGKMEKITCVGHTALDYIFNVEKFPEPNTSIQIPSARKYYGGAAANTAVGIKKLGVNSELLSCVGYDFKNSGYERYLKNLDINISKLYYSEEEETPKAWIFTDKDNNQITFFLWGAAKHYKELNPPNFNTEIVHIATGDPEFNLKCAKKAYGNNLVSFDPGQDLPQYSKEMLLEIIEHTNFLFMNKHEFERASNLLNFEIDDYLERVDALIVTKGSKGSVIYTKDKKIEIPCIKAGKVIDPTGAGDSYRAGFLSAYVKGYDLEKCGLIGAATASFVVEAKGCQTNLPTWDKVVERLEKHRI

Mass Spectrometry

Molecular mass is 33,937 Da. Determined by Electrospray.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L77117
EMBL· GenBank· DDBJ
AAB98396.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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