Q57506 · CYAA_BORBR

Function

function

Bifunctional adenylate cyclase toxin-hemolysin that plays a crucial role in host colonization. It causes whooping cough by acting on mammalian cells by elevating cAMP-concentration and thus disrupts normal cell function.

Calmodulin-sensitive adenylate cyclase

Adenylate cyclase that is activated by host intracellular calmodulin and catalyzes un-regulated conversion of ATP to cAMP, thereby impairing microbicidal functions of immune effector cells and inducing apoptosis of lung macrophages.

Hemolysin

Hemolysin that forms small cation-selective membrane channels, leading to hemolytic activity (By similarity).
The hemolytic activity of CyaA is weak compared with that of the HlyA of E.coli (By similarity).

Catalytic activity

Activity regulation

Calmodulin-sensitive adenylate cyclase

Activated by host calmodulin.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site349-356ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Molecular FunctionATP binding
Molecular Functioncalcium ion binding
Molecular Functioncalcium- and calmodulin-responsive adenylate cyclase activity
Molecular Functioncalmodulin binding
Molecular Functionchannel activity
Molecular Functiontoxin activity
Biological ProcesscAMP biosynthetic process
Biological Processkilling of cells of another organism

Keywords

Names & Taxonomy

Protein names

Gene names

    • Name
      cya
    • Synonyms
      cyaA
    • Ordered locus names
      BB0324

Organism names

Accessions

  • Primary accession
    Q57506
  • Secondary accessions
    • O05179

Proteomes

Subcellular Location

Secreted

Hemolysin

Host cell membrane
; Multi-pass membrane protein

Keywords

Phenotypes & Variants

Keywords

PTM/Processing

Features

Showing features for chain, lipidation.

TypeIDPosition(s)Description
ChainPRO_00000013181-312Calmodulin-sensitive adenylate cyclase
ChainPRO_0000001319313-1706Hemolysin
Lipidation860N6-palmitoyl lysine
Lipidation983N6-palmitoyl lysine

Post-translational modification

Released in a processed form.

Hemolysin

Palmitoylated at Lys-860 and Lys-983 by CyaC. The toxin only becomes active when modified in position Lys-983: palmitoylation is required for efficient membrane insertion and pore formation of the acylated Hemolysin chain.

Keywords

Family & Domains

Features

Showing features for region, repeat.

TypeIDPosition(s)Description
Region1-399A, catalytic
Region367-405Disordered
Region400-912B, Ala/Gly-rich
Region500-698Required for interaction with CyaC
Region913-1656C
Repeat1014-1031Hemolysin-type calcium-binding 1
Repeat1032-1049Hemolysin-type calcium-binding 2
Repeat1050-1067Hemolysin-type calcium-binding 3
Repeat1155-1172Hemolysin-type calcium-binding 4
Repeat1173-1190Hemolysin-type calcium-binding 5
Repeat1279-1296Hemolysin-type calcium-binding 6
Repeat1297-1314Hemolysin-type calcium-binding 7
Repeat1315-1332Hemolysin-type calcium-binding 8
Repeat1335-1352Hemolysin-type calcium-binding 9
Repeat1411-1428Hemolysin-type calcium-binding 10
Repeat1429-1446Hemolysin-type calcium-binding 11
Repeat1447-1464Hemolysin-type calcium-binding 12
Repeat1468-1484Hemolysin-type calcium-binding 13
Repeat1537-1554Hemolysin-type calcium-binding 14
Repeat1555-1572Hemolysin-type calcium-binding 15
Repeat1573-1590Hemolysin-type calcium-binding 16
Repeat1603-1620Hemolysin-type calcium-binding 17
Region1657-1706D, Asp/Gly-rich

Domain

The Gly-rich region is probably involved in binding calcium, which is required for target cell-binding or cytolytic activity.

Sequence similarities

In the N-terminal section; belongs to the adenylyl cyclase class-2 family.
In the C-terminal section; belongs to the RTX prokaryotic toxin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    1,706
  • Mass (Da)
    177,056
  • Last updated
    2003-09-19 v4
  • Checksum
    AF51E8270EA8A68F
MQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNATLMFRLVNPHSTSLIAEGVATKGLGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDVNSSLAHGHTAVDLTLSKERLDYLRQAGLVTGMADGVVASNHAGYEQFEFRVKETSDGRYAVQYRRKGGDDFEAVKVIGNAAGIPLTADIDMFAIMPHLSNFRDSARSSVTSGDSVTDYLARTRRAASEATGGLDRERIDLLWKIARAGARSAVGTEARRQFRYDGDMNIGVITDFELEVRNALNRRAHAVGAQDVVQHGTEQNNPFPEADEKIFVVSATGESQMLTRGQLKEYIGQQRGEGYVFYENRAYGVAGKSLFDDGLGAAPGVPGGRSKSSPDVLETVPASPGLRRPSLGAVERQDSGYDSLDGVGSRSFSLGEVSDMAAVEAAELEMTRQVLHAGARQDDAEPGVSGASAHWGQRALQGAQAVAAAQRLVHAIALMTQFGRAGSTNTPQEAASLSAAVFGLGEASSAVAETVSGFFRGSSRWAGGFGVAGGAMALGGGIAAAVGAGMSLTDDAPAGQKAAAGAEIALQLTGGTVELASSIALALAAARGVTSGLQVAGASAGAAAGALAAALSPMEIYGLVQQSHYADQLDKLAQESSAYGYEGDALLAQLYRDKTAAEGAVAGVSAVLSTVGAAVSIAAAASVVGAPVAVVTSLLTGALNGILRGVQQPIIEKLANDYARKIDELGGPQAYFEKNLQARHEQLANSDGLRKMLADLQAGWNASSVIGVQTTEISKSALELAAITGNADNLKSADVFVDRFIQGERVAGQPVVLDVAAGGIDIASRKGERPALTFITPLAAPGEEQRRRTKTGKSEFTTFVEIVGKQDRWRIRDGAADTTIDLAKVVSQLVDANGVLKHSIKLEVIGGDGDDVVLANASRIHYDGGAGTNTVSYAALGRQDSITVSADGERFNVRKQLNNANVYREGVATQKTAYGKRTENVQYRHVELARVGQLVEVDTLEHVQHIIGGAGNDSITGNAHDNFLAGGAGDDRLDGGAGNDTLVGGEGHNTVVGGAGDDVFLQDLGVWSNQLDGGAGVDTVKYNVHQPSEERLERMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSSLNDSIAGDDRDNELWGDDGNDTIHGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDDIDGGAGLDTVDYSAMIHAGKIVAPHEYGFGIEADLSEGWVRKAARRGMDYYDSVRSVENVIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLLFGGDGNDMLYGDAGNDTLYGGLGDDTLEGGAGNDWFGQTPAREHDVLRGGAGVDTVDYSQAGAHAGVATGRIGLGILADLGAGRVDKLGEAGSSAYDTVSGIENVVGTELADRITGDAQANVLRGAGGADVLAGGEGDDVLLGGDGDDQLSGDAGRDRLYGEAGDDWFFQDAANAGNLLDGGDGNDTVDFSGPGRGLDAGAKGVFLSLGKGFASLMDEPETSNVLRHIENAVGSVRDDVLIGDAGANVLNGLAGNDVLSGGAGDDVLLGDEGSDLLSGDAGNDDLFGGQGDDTYLFGAGYGHDTIYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRDADHRVEAIHAANQAIDPAGIEKLVEAMAQYPDPGAAAAAPPAARVPDTLMQSLAVNWR

Sequence caution

The sequence CAE30822.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict292in Ref. 1; CAA85481
Sequence conflict371in Ref. 1; CAA85481
Sequence conflict546-547in Ref. 1; CAA85481

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z37112
EMBL· GenBank· DDBJ
CAA85481.2
EMBL· GenBank· DDBJ
Genomic DNA
BX640437
EMBL· GenBank· DDBJ
CAE30822.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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