Q56061 · SIFA_SALTY

Function

function

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella-induced filaments (Sifs), which are filamentous structures containing lysosomal membrane glycoproteins within epithelial cells. Sif formation is concomitant with intracellular bacterial replication.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell cytoplasm
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Biological Processregulation of protein localization
Biological Processsymbiont-mediated perturbation of host microtubule cytoskeleton
Biological Processsymbiont-mediated perturbation of host small GTPase-mediated signal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Secreted effector protein SifA

Gene names

    • Name
      sifA
    • Ordered locus names
      STM1224

Organism names

Accessions

  • Primary accession
    Q56061

Proteomes

Subcellular Location

Note: Secreted via type III secretion system 2 (SPI-2 T3SS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells.

Keywords

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene do not produce Sifs in epithelial cells and show attenuated virulence in mice.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis130Loss of interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-131.
Mutagenesis131Alters interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-130.
Mutagenesis197Fails to induce host endosomal tubulation; when associated with A-201.
Mutagenesis201Fails to induce host endosomal tubulation; when associated with A-197.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000977601-336Secreted effector protein SifA

Proteomic databases

Expression

Induction

Very highly expressed in host macrophages and when grown in an acidic environment; repressed by H-NS and induced by SsrB (PubMed:12949164, PubMed:19858298, PubMed:21059643).
Repressed in conditions of high calcium and by osmotic stress (PubMed:12949164).

Interaction

Subunit

Interacts with host PLEKHM2. Interacts with SseJ; the interaction is indirect.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO Q56061PLEKHM2 Q8IWE53EBI-10765408, EBI-726484

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-330Interaction with host PLEKHM2

Domain

Domains throughout the protein, and not only the N-terminus, are required for secretion and translocation. Both N- and C-terminal domains are also required for formation of tubules.

Sequence similarities

Belongs to the Sif family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    336
  • Mass (Da)
    38,498
  • Last updated
    1997-11-01 v1
  • Checksum
    83C6185148A774B9
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAPTRERLTEIFFELKELACASQRDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFSCEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPLPSTLCLNPAGAPILSVPLDNIEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMAMCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFHAIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U51867
EMBL· GenBank· DDBJ
AAA97467.1
EMBL· GenBank· DDBJ
Genomic DNA
AE006468
EMBL· GenBank· DDBJ
AAL20153.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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