Q55CT1 · NCPR_DICDI
- ProteinNADPH--cytochrome P450 reductase
- GeneredB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids667 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity
- 2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP+ + H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD per monomer.
Note: Binds 1 FMN per monomer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 164-173 | FMN (UniProtKB | ChEBI) | |||
Binding site | 199 | FMN (UniProtKB | ChEBI) | |||
Binding site | 297 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 468-470 | FAD (UniProtKB | ChEBI) | |||
Binding site | 484-487 | FAD (UniProtKB | ChEBI) | |||
Binding site | 527 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 586-587 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 592-596 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 666 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Molecular Function | oxidoreductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH--cytochrome P450 reductase
- EC number
- Short namesCPR ; P450R
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionQ55CT1
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-8 | Lumenal | |||
Transmembrane | 9-29 | Helical | |||
Topological domain | 30-667 | Cytoplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000351223 | 1-667 | NADPH--cytochrome P450 reductase | ||
Proteomic databases
Expression
Developmental stage
Present during growth and development.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 72-215 | Flavodoxin-like | |||
Domain | 277-511 | FAD-binding FR-type | |||
Sequence similarities
Belongs to the NADPH--cytochrome P450 reductase family.
In the N-terminal section; belongs to the flavodoxin family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length667
- Mass (Da)76,193
- Last updated2005-05-24 v1
- MD5 ChecksumF3BED16DF7877725DE8ADFA03A62AC49
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFI02000005 EMBL· GenBank· DDBJ | EAL72306.1 EMBL· GenBank· DDBJ | Genomic DNA |