Q55C16 · UBA1_DICDI

Function

function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.

Catalytic activity

  • ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.
    EC:6.2.1.45 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site438ATP (UniProtKB | ChEBI)
Binding site464ATP (UniProtKB | ChEBI)
Binding site475ATP (UniProtKB | ChEBI)
Binding site488ATP (UniProtKB | ChEBI)
Binding site536-537ATP (UniProtKB | ChEBI)
Active site592Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular Functionubiquitin activating enzyme activity
Biological ProcessDNA damage response
Biological Processprotein ubiquitination
Biological Processubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-like modifier-activating enzyme 1
  • EC number
  • Alternative names
    • Ubiquitin-activating enzyme E1

Gene names

    • Name
      uba1
    • Synonyms
      uae1
    • ORF names
      DDB_G0270272

Organism names

Accessions

  • Primary accession
    Q55C16

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003283631-1017Ubiquitin-like modifier-activating enzyme 1

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for repeat, region, compositional bias.

Type
IDPosition(s)Description
Repeat26-1631-1
Region26-5712 approximate repeats
Repeat419-5711-2
Compositional bias765-785Polar residues
Region765-788Disordered

Sequence similarities

Belongs to the ubiquitin-activating E1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,017
  • Mass (Da)
    113,745
  • Last updated
    2005-05-24 v1
  • MD5 Checksum
    5858BD92096E5B40800B66658E3D8C80
MSKPMDVEQEPKIDDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKEGIVTVVEEQKLQLLDGDLVTFKEVNGMSALNDLPPQKIKTISPLTFSIGDTTNLPPYTSGGYVTEVKQPKVVDFKPLKNILESGENIFITDDFKFTQPTNLLAGFQAIHKFAEKNKHMPRPHNKEDANAVIEIAKGLLKKPDDELDEKMITQLSFGAQGDIVPMQAILGGITAQEVLKACSGKFTPIHQLAFFDSVECLPEDLETLPEEEFQPIGSRYDGQIITFGKTLQNKIENLNYFLVGAGAIGCEMLKNFAMMGLGAGPKGLVHVTDMDTIEKSNLNRQFLFRSSDIQQLKSQTAANAVRVMNPDLNVKAYSLRVGPDTESHYNEEFFNSLDGVCNALDNVEARLYMDSQCVYYGKPLLESGTLGTKGNTQVVVPHLTESYSSSRDPPEKGIPVCTLHNFPNAIEHTIQWARDTFEGLFKNNADNVNSYLTNPAYVQSLKTQNPFVRLETLASIKASLMDRPLDFNQCIAWARLKFEEYFNNNIEQLLYNFPKDMVTTTGTPFWSGPKRAPTPLKFDVENPLHLEFIVAAANLRAFNYGIKAETNIEVIQKQAANVIVPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLEICCNDTETGDDVDVPSVRYKYN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias765-785Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAFI02000005
EMBL· GenBank· DDBJ
EAL72486.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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