Q55C16 · UBA1_DICDI
- ProteinUbiquitin-like modifier-activating enzyme 1
- Geneuba1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1017 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.
Miscellaneous
There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 438 | ATP (UniProtKB | ChEBI) | |||
Binding site | 464 | ATP (UniProtKB | ChEBI) | |||
Binding site | 475 | ATP (UniProtKB | ChEBI) | |||
Binding site | 488 | ATP (UniProtKB | ChEBI) | |||
Binding site | 536-537 | ATP (UniProtKB | ChEBI) | |||
Active site | 592 | Glycyl thioester intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | centrosome | |
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ubiquitin activating enzyme activity | |
Biological Process | DNA damage response | |
Biological Process | protein ubiquitination | |
Biological Process | ubiquitin-dependent protein catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin-like modifier-activating enzyme 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionQ55C16
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000328363 | 1-1017 | Ubiquitin-like modifier-activating enzyme 1 | ||
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for repeat, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Repeat | 26-163 | 1-1 | |||
Region | 26-571 | 2 approximate repeats | |||
Repeat | 419-571 | 1-2 | |||
Compositional bias | 765-785 | Polar residues | |||
Region | 765-788 | Disordered | |||
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,017
- Mass (Da)113,745
- Last updated2005-05-24 v1
- MD5 Checksum5858BD92096E5B40800B66658E3D8C80
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 765-785 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFI02000005 EMBL· GenBank· DDBJ | EAL72486.1 EMBL· GenBank· DDBJ | Genomic DNA |