Q55BH9 · DGAT1_DICDI

Function

function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.

Catalytic activity

  • an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.20 (UniProtKB | ENZYME | Rhea)
  • all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.76 (UniProtKB | ENZYME | Rhea)
  • 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA
    This reaction proceeds in the forward direction.
  • 1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA
    This reaction proceeds in the forward direction.
  • all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl hexadecanoate + CoA
    This reaction proceeds in the forward direction.
  • 1-O-(9Z-octadecenyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + CoA
    This reaction proceeds in the forward direction.
  • 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-octadecenoyl)glycerol + CoA
    This reaction proceeds in the forward direction.
  • 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA
    This reaction proceeds in the forward direction.
  • 1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H+ = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O
    This reaction proceeds in the forward direction.
  • 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoA
    This reaction proceeds in the forward direction.
  • hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O-dihexadecanoyl-1,2-hexadecanediol + 2 CoA
    This reaction proceeds in the forward direction.
  • hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl hexadecanoate + CoA
    This reaction proceeds in the forward direction.
  • 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA
    This reaction proceeds in the forward direction.
  • 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA
    This reaction proceeds in the forward direction.
  • hexadecan-1-ol + hexadecanoyl-CoA = hexadecanyl hexadecanoate + CoA
    This reaction proceeds in the forward direction.
  • 13-cis-retinol + hexadecanoyl-CoA = 13-cis-retinyl hexadecanoate + CoA
    This reaction proceeds in the forward direction.
  • 1,3-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA
    This reaction proceeds in the forward direction.
  • 2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA
    This reaction proceeds in the forward direction.

Pathway

Lipid metabolism; glycerolipid metabolism.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site532

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functiondiacylglycerol O-acyltransferase activity
Molecular Functionretinol O-fatty-acyltransferase activity
Biological Processether lipid biosynthetic process
Biological Processglycerol metabolic process
Biological Processlipid droplet formation
Biological Processmonoacylglycerol biosynthetic process
Biological Processtriglyceride biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Diacylglycerol O-acyltransferase 1
  • EC number
  • Alternative names
    • Acyl-CoA retinol O-fatty-acyltransferase (ARAT; Retinol O-fatty-acyltransferase) (EC:2.3.1.76
      ) . EC:2.3.1.76 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      dgat1
    • ORF names
      DDB_G0271342

Organism names

Accessions

  • Primary accession
    Q55BH9

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane217-237Helical
Transmembrane254-274Helical
Transmembrane306-326Helical
Transmembrane399-419Helical
Transmembrane449-469Helical
Transmembrane520-540Helical
Transmembrane545-565Helical
Transmembrane570-590Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003774881-617Diacylglycerol O-acyltransferase 1

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, motif.

TypeIDPosition(s)Description
Compositional bias1-38Polar residues
Region1-52Disordered
Compositional bias95-146Polar residues
Region95-186Disordered
Compositional bias161-186Polar residues
Motif477-483FYXDWWN motif

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    617
  • Mass (Da)
    71,946
  • Last updated
    2009-06-16 v2
  • Checksum
    DCD8FD193A3023D7
MEPIPPSNGNKNNSMDKQPQQPQQPQQQQQQQQQQRRDQRNSKLNELNETERVRNRFISHEFHKLDRTKSRIDAPKISFSDSESESDSEFFLAKRNTNNNNQNNTSPTFSSANGKQSNLTQRKINTQIQSKQPTNNNVQPLTDDEGTINHSNHHHHHHNQNNNGNNNNNNNNNNNNNKISTPPKQEEKMTMNGLFTLRPSILSSESNGSSYRGFLNLLLILLITASFRLVILNHLLYGIRINLDLYKISEYHRWPGVMISLMINLFIIAAYLIEKAAAKQLLPDRICYLLRIINCAAVIIVPSGSIIAFSPNPASGIIVMILICTFSMKIISYAYENSKQRKLNPDNKKFVIDPTNTSIYPNNLSLRSTYWFMLVPTLVYQLSYPRSPKIRKGYLLRRIVEALSLSLLILWMVNQYMLPLVQNSIEPLEKIDIVLIVERIMKLSLPNLYVWLLGFYVFFHLYLNIVAEITRFGDREFYRDWWNSTGLDYFWRTWNMPVHHWMVVLIYTPMRRRGFSKNMGYFMCFFVSAIFHELVISIPFHSLKLWGFFGIMSQMVLIALTKNLMNGRNLGNVIFWISIVLGQPLVVLLYYRNFVLENPEWYRNVEPPTSPPVMPFY

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-38Polar residues
Compositional bias95-146Polar residues
Compositional bias161-186Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAFI02000006
EMBL· GenBank· DDBJ
EAL71801.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp