Q55643 · ISPH_SYNY3

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site39[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site69(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site69dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site69isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site130[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site158(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site158dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site158isopentenyl diphosphate (UniProtKB | ChEBI)
Active site160Proton donor
Binding site223(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site261[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site290(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site290dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site290isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site291(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site291dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site291isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site292(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site292dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site292isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site352(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site352dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site352isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Synonyms
      lytB
    • Ordered locus names
      slr0348

Organism names

Accessions

  • Primary accession
    Q55643
  • Secondary accessions
    • Q55066

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001288801-3794-hydroxy-3-methylbut-2-enyl diphosphate reductase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    379
  • Mass (Da)
    42,415
  • Last updated
    2000-12-01 v2
  • Checksum
    4CD0167AF5D4957E
MGVMNTEYQSHLIQEIRQNNYRLERGDVTILLAEAFGFCWGVERAVAMAYETRQHFPGDRLWITNEIIHNPSVNQRLREMEVNFIDVVNGEKDFSGVAKGDVVILPAFGASVEEMQLLNDRECTIVDTTCPWVSKVWNSVEKHKKKEHTSIIHGKYNHEETIATSSFAGTYLIVLNMAEAQKVCDYILHGGDRQEFLDYFANAHSAGFDPDQDLVRLGVANQTTMLKSETEMIGKLFEKTLLQKYGPIELNNHFMSFNTICDATQERQDAMFDLVEEDLSLMVVIGGFNSSNTTHLQEIAVEHGIPSVHIDSGDRIGPGNRVEHKPLGKDLEVLEPWLPAGKITVGVTSGASTPDKVVEEVMKKILAIKEAQPVLEIAG

Sequence caution

The sequence BAA10159.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U38915
EMBL· GenBank· DDBJ
AAB72119.1
EMBL· GenBank· DDBJ
Genomic DNA
BA000022
EMBL· GenBank· DDBJ
BAA10159.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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