Q54QG9 · UBA3_DICDI
- ProteinNEDD8-activating enzyme E1 catalytic subunit
- Geneuba3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids442 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Regulatory subunit of the dimeric uba3-nae1 E1 enzyme. E1 activates nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1 finally transfers nedd8 to the catalytic cysteine of ube2m (By similarity).
Miscellaneous
Arg-192 acts as a selectivity gate, preventing misactivation of ubiquitin by this nedd8-specific E1 complex.
Nae1 and uba3 correspond to the N-terminal and the C-terminal part of yeast uba3. In yeast the two subunits form a single polypeptide chain.
Catalytic activity
Pathway
Protein modification; protein neddylation.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 80-104 | ATP (UniProtKB | ChEBI) | |||
Binding site | 128-151 | ATP (UniProtKB | ChEBI) | |||
Site | 192 | Determines specificity for NEDD8 | |||
Active site | 218 | Glycyl thioester intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | NEDD8 activating enzyme activity | |
Biological Process | protein neddylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNEDD8-activating enzyme E1 catalytic subunit
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionQ54QG9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000330897 | 1-442 | NEDD8-activating enzyme E1 catalytic subunit | ||
Proteomic databases
Interaction
Subunit
Heterodimer of uba3 and nae1. The complex binds nedd8 and ube2m.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length442
- Mass (Da)49,301
- Last updated2005-05-24 v1
- MD5 Checksum76B0482DAE22D3FF3047176AE5C653CB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFI02000057 EMBL· GenBank· DDBJ | EAL65560.1 EMBL· GenBank· DDBJ | Genomic DNA |