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Q54QG9 · UBA3_DICDI

Function

function

Regulatory subunit of the dimeric uba3-nae1 E1 enzyme. E1 activates nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1 finally transfers nedd8 to the catalytic cysteine of ube2m (By similarity).

Miscellaneous

Arg-192 acts as a selectivity gate, preventing misactivation of ubiquitin by this nedd8-specific E1 complex.
Nae1 and uba3 correspond to the N-terminal and the C-terminal part of yeast uba3. In yeast the two subunits form a single polypeptide chain.

Catalytic activity

  • ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.
    EC:6.2.1.64 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein neddylation.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site80-104ATP (UniProtKB | ChEBI)
Binding site128-151ATP (UniProtKB | ChEBI)
Site192Determines specificity for NEDD8
Active site218Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionNEDD8 activating enzyme activity
Biological Processprotein neddylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NEDD8-activating enzyme E1 catalytic subunit
  • EC number
  • Alternative names
    • NEDD8-activating enzyme E1C
    • Ubiquitin-activating enzyme E1C
    • Ubiquitin-like modifier-activating enzyme 3 (Ubiquitin-activating enzyme 3)

Gene names

    • Name
      uba3
    • Synonyms
      ube1c
    • ORF names
      DDB_G0283891

Organism names

Accessions

  • Primary accession
    Q54QG9

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003308971-442NEDD8-activating enzyme E1 catalytic subunit

Proteomic databases

Interaction

Subunit

Heterodimer of uba3 and nae1. The complex binds nedd8 and ube2m.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    442
  • Mass (Da)
    49,301
  • Last updated
    2005-05-24 v1
  • MD5 Checksum
    76B0482DAE22D3FF3047176AE5C653CB
METMDTSVDLPGRWIDIEKIIKRTGPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCEILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTPHKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGGTEGFKGQARVILPKISSCFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDATLEKPFDPKQFDNDNPDHMNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAIIAAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSNTLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQGLLHQSTVPNLEKTLSELNVQEDDEITITDPALPGNLAVRMRIKYTS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAFI02000057
EMBL· GenBank· DDBJ
EAL65560.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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