Q54NJ8 · HPRT_DICDI

Function

function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
55.5 μMhypoxanthine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
34.3 nmol/min/mg
When guanine is used as the substrate, the rate of nucleotide formation is 50% that with hypoxanthine as the substrate.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site40GMP (UniProtKB | ChEBI)
Binding site99-107GMP (UniProtKB | ChEBI)
Active site103Proton acceptor
Binding site131GMP (UniProtKB | ChEBI)
Binding site159GMP (UniProtKB | ChEBI)
Binding site159Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionguanine phosphoribosyltransferase activity
Molecular Functionhypoxanthine phosphoribosyltransferase activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleotide binding
Biological ProcessGMP salvage
Biological Processguanine salvage
Biological Processhypoxanthine metabolic process
Biological ProcessIMP salvage
Biological Processpurine ribonucleoside salvage

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Hypoxanthine-guanine phosphoribosyltransferase
  • EC number
  • Short names
    HGPRT; HGPRTase

Gene names

    • Name
      hprT
    • ORF names
      DDB_G0285193

Organism names

Accessions

  • Primary accession
    Q54NJ8

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003283341-180Hypoxanthine-guanine phosphoribosyltransferase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    180
  • Mass (Da)
    20,536
  • Last updated
    2005-05-24 v1
  • Checksum
    68D542EEEE7B6D2F
MSREVIFTEKEIKERVSELGRQITQDYKDSKNLVLVGILKGSFVFMSDLVRSIHLPNTNVSLEFMSISSYGAETSSSGVIRIMMDLRTSIEGKDVLIIEDIVDSGLTLKHLMELLNHRNPNSLHTAVLLRKKEGLKVEVPVKYMGFDIPMVFIIGYGLDFAENYRELPYLGELKEECYKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAFI02000075
EMBL· GenBank· DDBJ
EAL64853.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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