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Q54150 · IPAJ_SHIFL

  • Protein
    Cysteine protease IpaJ
  • Gene
    ipaJ
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Virulence factor that eliminates N-myristoyl protein modifications in infected host cells. Acts as a cysteine protease that cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3 of human ARF1, leading to the elimination of the myristoyl group and alteration of protein trafficking in host cell. Could also cleave an array of N-myristoylated host proteins involved in cellular growth, signal transduction, autophagasome maturation and organelle function.

Features

Showing features for active site.

125920406080100120140160180200220240
TypeIDPosition(s)Description
Active site64
Active site206
Active site218

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell cytoplasm
Molecular Functionpeptidase activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cysteine protease IpaJ
  • EC number
  • Alternative names
    • Effector protein IpaJ
    • Invasion plasmid antigen J

Gene names

    • Name
      ipaJ
    • ORF names
      pWR501_0130
    • Ordered locus names
      CP0122

Encoded on

  • Plasmid pWR100
  • Plasmid pWR501
  • Plasmid pCP301
  • Plasmid pINV_F6_M1382

Organism names

  • Taxonomic identifier
  • Strains
    • M90T / Serotype 5a
    • M1382 / Serotype 6
    • 301 / Serotype 2a
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella

Accessions

  • Primary accession
    Q54150
  • Secondary accessions
    • Q7BEM0
    • Q8VSI0

Proteomes

Subcellular Location

Secreted
Host cytoplasm
Note: Secreted via Mxi-Spa type III secretion system (T3SS), and delivered into the host cytoplasm.

Keywords

Phenotypes & Variants

Disruption phenotype

Mutants are not compromised in their ability to invade cultured epithelial cells or to form plaques on BHK cell monolayers.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis64Lack of activity.
Mutagenesis206Lack of activity.
Mutagenesis218Lack of activity.

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004233951-259Cysteine protease IpaJ

Proteomic databases

Expression

Induction

Expression is temperature-independent.

Family & Domains

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    259
  • Mass (Da)
    28,807
  • Last updated
    1996-11-01 v1
  • MD5 Checksum
    B05721C31B7428810681059A71CDFD44
MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNENRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNRCERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVISFIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMDPAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U32973
EMBL· GenBank· DDBJ
AAA84386.1
EMBL· GenBank· DDBJ
Genomic DNA
AL391753
EMBL· GenBank· DDBJ
CAC05797.1
EMBL· GenBank· DDBJ
Genomic DNA
AF348706
EMBL· GenBank· DDBJ
AAK18440.1
EMBL· GenBank· DDBJ
Genomic DNA
AY206439
EMBL· GenBank· DDBJ
AAP78985.1
EMBL· GenBank· DDBJ
Genomic DNA
AF386526
EMBL· GenBank· DDBJ
AAL72344.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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