Q53AM8 · Q53AM8_9VIRU

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = L-aspartyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + phosphate + H+
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • N7-methyl-GTP + L-histidyl-[protein] = N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + diphosphate
    This reaction proceeds in the forward direction.
  • N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H+
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1025For cysteine protease nsP2 activity
Active site1095For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular Functionpoly(A) RNA polymerase activity
Molecular Functionpolynucleotide 5'-phosphatase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • CMS1
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Togaviridae > Alphavirus

Accessions

  • Primary accession
    Q53AM8

Subcellular Location

Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Interacts with RNA-directed RNA polymerase nsP4. Interacts with mRNA-capping enzyme nsP1. Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain21-257Alphavirus-like MT
Domain1016-1374Peptidase C9
Domain1393-1562Macro
Region1737-1884Disordered
Compositional bias1767-1781Polar residues
Compositional bias1797-1816Polar residues
Compositional bias1854-1868Polar residues
Region1900-1983Disordered
Compositional bias1906-1944Polar residues
Compositional bias1966-1983Polar residues
Domain2326-2441RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    2,569
  • Mass (Da)
    282,409
  • Last updated
    2005-05-24 v1
  • MD5 Checksum
    6BBB4A24F6FFBDC57B422025E2098137
LPADHPALNQFQTAFPGFEVVASNRSSNDHAAARAFSHLATKWIERDIDGRQVIVADIGSAPARRVGAPDNVTYHSVCPRKCAEDPERLASYARKLVRAVERGDGHLVSDRITDLKDVLENPDTSLETTSICLNDDVSCKVKADIAVYQDVYAVDAPSTIYAQADKGTRVVYWIGFEPFVFHTDAMAGSFPLYDANWSDSAVLAAKNLPLCYSGLSEDSINWRFRFRDKPLVPSGEIHYSVGSTHYVEDRDKLKSWHLPSTFHFVAPNKYTCRCDTVVSCGGYVVKKITICEGIVGRPANEELATSYHRDGVVVTKFSDTINHEQVSFPVVTYIPAVICDQMTAMTANPVKYPDVVKLLVGLNQRIVVNGTTVRNVNSMDNSLIPVFARALCSWADEVRRDMEDEQDMYGITSVTTWICICRAYDKRQQHTFYRRPKQSSGIYVPAKFTGSLRASLSATYLNLPLKQLLLNTLKRAIKPMDQAIADETEARAHDAAEVHELTEEEGRQQAANPSYIADVLDQDDVEEVDDVMSDVDLGEEDGVGATIIDCHRGTVKVITAFSDNTMGEYLVLSPVTVLRTRKLAVLLGPLADEVMQYVHKGRTGRYAIEKNNLKVLIPTGVSLKTAHFQALAESATLTYNDYLFTCRTLDQLATRGPAKNTDEVYYKLVDAAKAKDEYVYELSSKQCVKKEDATGTVLQGDICNPPYHQFAYEALRKRPAHTHDVHTIGIYGVPGAGKTAIITTEVTTRDLVASGKKENCEDIKRCVLERRGLKIAARTVDSLLYGAYRGAVDTLYVDEAYACHSGTLLALIAAVRPTGKVVLCGDPKQVGCVNQLQMRMHYNHEISDHVLRKNISRRCTHTLTAIVSNLNYEGRMKTTNPCKKPVLIDTTGSTKPDKEALVLTCFRGWVKDLKILYPHNELMTAAASQGLTREKVYAVRCRVTSNPLYEPTSEHITVLLTRTNDELVWKTLPNDPLIPILSKPPKGDYSATMEDWEDEHNGILAALREACVPRMNFAHGKRNTCWAVTSSRVLHEAGVLITPEDYNRIFPAFREDKPHSALAALDAVATLVWGLDTSSGILSGKGSFMRLENSHWSNSNRGYEYGLNLEALEGYEIANPRMIKALKQRRGRECYDTETGKLVPMDPARVQVPINRVVPHVLVDTSAAAKPGFLENRLTVDRWDQVHSFKTRAAVKFAELTKRVSYNSVLDLGAAPGGVTDYCVKKGKTVTCVSEQWDSKPRGAVVVTADINGPLNNLGIFDLVFCDAAGPRRYHHYAQCEDHAVLFTSACKHGVERTAKGGVFIVKAYGMADRRTERAVECTARYFKSVSVEKPVSSRITNVEVFFKFSGRCRPLARSIAHLGPQLTDIYARTRKAYKMLARGGVADKVKVAEILNSMVGAAPGYRVLNRNIITAEEEVLVNAANSNGRPGDGVCGALYGAFGDAFPNGAIGAGNAVLVRGLEATIIHAAGADFREVDEETGARQLRAAYRAAANLVTANGITSAAIPLLSTHIFSNGRNRLEQSFGALVEAFDTTECDVTIYCLANNMAVRIQQLIDDHAREEFDEEVVVEEEEEHEADAMSDTETLSSFGDETVWVPKHSTLAGRPGYSASYGDRRSLFVGTKFHRAAVAMSSIEAAWPKTKEANAKLIEYIRGQHLVDVLKSCPVNDIPVGRPPSSLPCGCIYAMTPERVTVLKQRPQEGFVVCSAFKLPLTNIQDVTKVECTVRAPAEEPRPVRYLQERRPVQAAVGQPRPATVAARVAASHAASGTSTATSRHTPAPGSVRVRLLPPRDGTVSRSSRTGSQSSVTSSAGSILPVPRRAPVTPAASLAGSVHGHSVRSAPAMRAASTGARSVRSVQSGSAGHRTDVASVAGSAGLPRGLTRDQFGAVRARARRDLELEGSEHGSQTSFRSGSLMVESTASGYSQRSDDQDTGSEPSSRGASVRTRRRGQRDGPGGYIFSSDQGTAHLSQHNTQTNNTTEVLMRTSVLPSNDHGTPDLPAETKKKLAYQMRPTQKNKSRYLSAKVHNMKHKIVQCLQRGAGHYLREQHALPLWKNTFPKPRYSDACVVKFESVNTAIVAANMFIGCNYPTLSSFGVTDKYDAYLDMVDGLNCNLDTVTFDPAKVRSLPKKSEYNQPLIQSQVPGPMASTLQSILMAATKRNCNVTQMRELPTLDSAAMNVEAFKKFACKDTDLWTEFAEKPVRLSPGQIEEYVFHLQGAKANVMHSRVEAVCPNLSEVAMDRFTLDMKRDVKVTPGTKHVEERPKVQVIQAADPMATAYLCAIHRELVRRLKAVLKPSIHVLFDMSSEDFDAIVGHGMKLGDKVLETDISSFDKSQDQAMAVTALMLLEDLGVEEDLLTLIEASFGDITSVHLPTGTRFQFGSMMKSGLFLTLFVNTLLNITIAARVLREQLADTRCAAFIGDDNVITGVVSDDMMVARCASWLNMEVKIMDMEIGNRSPYFCGGFLLLDTVTGTVSRVSDPVKRLMKMGKPALNDPETDVDRCRALREEVESWYRVGIQWPLQVAVATRYGVNHLPLATMAMATLAQDLRSYLGARGEYVSLYA

Features

Showing features for non-terminal residue, compositional bias.

Type
IDPosition(s)Description
Non-terminal residue1
Compositional bias1767-1781Polar residues
Compositional bias1797-1816Polar residues
Compositional bias1854-1868Polar residues
Compositional bias1906-1944Polar residues
Compositional bias1966-1983Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY604236
EMBL· GenBank· DDBJ
AAU01397.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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