Q53353 · LSDX1_SPHPI

Function

function

Catalyzes the cleavage of the interphenyl double bond (C alpha-C beta) of lignin-derived polyphenolic diaryl-propane type compounds (Stilbene).

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: 1 Fe2+ ion per subunit.

Activity regulation

Activity is high with beta-1 type stilbene and minimal with beta-5 type stilbene. A 4-hydroxyl group and trans-stilbene structure is essential for the binding of substrates to the enzyme. Inhibited by N-benzylideneaniline-hydroxy and -methoxy derivatives and N-(4-hydroxybenzyl)-3-methoxyaniline but not N-benzylideneaniline. Fluorinated olefin (Z)-1-fluoro-1(4-hydroxyphenyl)-2-phenylethylene is also a potent inhibitor. Imine linkage instead of ethylene bridge in the substrate enhances the inhibitory activity.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
11 μM1,2-bis(4-hydroxy-3-methoxyphenyl)-ethylene
110 μMoxygen
8.3 μM4-hydroxystilbene

pH Dependence

Optimum pH is 8.5. Active from 5.5-10.5.

Temperature Dependence

Optimum temperature is 30 degrees Celsius. Stable at 4 degrees Celsius with minimal loss of activity.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site167Fe cation (UniProtKB | ChEBI); catalytic
Binding site218Fe cation (UniProtKB | ChEBI); catalytic
Binding site282Fe cation (UniProtKB | ChEBI); catalytic
Binding site472Fe cation (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular Functioncarotenoid dioxygenase activity
Molecular Functioniron ion binding
Molecular Functionlignostilbene alpha beta-dioxygenase activity
Biological Processcarotene catabolic process
Biological Processlignin catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lignostilbene-alpha,beta-dioxygenase isozyme I
  • EC number
  • Short names
    LSD-I

Organism names

Accessions

  • Primary accession
    Q53353

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004169402-485Lignostilbene-alpha,beta-dioxygenase isozyme I

Interaction

Subunit

Homodimer of two alpha subunits.

Family & Domains

Sequence similarities

Belongs to the carotenoid oxygenase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    485
  • Mass (Da)
    54,436
  • Last updated
    1996-11-01 v1
  • Checksum
    71A672E375D29A1C
MAHFPQTPGFSGTLRPLRIEGDILDIEIEGEVPPQLNGTFHRVHPDAQFPPRFEDDQFFNGDGMVSLFRFHDGKIDFRQRYAQTDKWKVERKAGKSLFGAYRNPLTDDASVQGMIRGTANTNVMVHAGKLYAMKEDSPCLIMDPLTLETEGYTNFDGKLQSQTFCAHPKIDPVTGNLCAFAYGAKGLMTLDMAYIEISPTGKLLKEIPFQNPYYCMMHDFGVTEDYAVFAVMPLLSSWDRLEQRLPFFGFDTTLPCYLGILPRNGDARDLRWFKTGNCFVGHVMNAFNDGTKVHIDMPVSRNNSFPFFDVHGAPFDPVAGQGFLTRWTVDMASNGDSFEKTERLFDRPDEFPRIDERYATRAYRHGWMLILDTEKPYEAPGGAFYALTNTLGHIDLATGKSSSWWAGPRCAIQEPCFIPRSPDAPEGDGYVIALVDDHVANYSDLAIFDAQHVDQGPIARAKLPVRIRQGLHGNWADASRLAVAA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
S65040
EMBL· GenBank· DDBJ
AAC60447.2
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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