Q53005 · 4HBCL_RHOPA
- Protein4-hydroxybenzoate--CoA/benzoate--CoA ligase
- GenehbaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids539 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the ligation of 4-hydroxybenzoate, benzoate or cyclohex-1,4-dienecarboxylate and CoA at the expense of ATP. The enzyme shows low activity towards cyclo-2,5-dienecarboxylate, 4-fluorobenzoate, 4-chlorobenzoate and 2-methoxybenzoate.
Catalytic activity
- 4-hydroxybenzoate + ATP + CoA = 4-hydroxybenzoyl-CoA + AMP + diphosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
400 μM | benzoate | |||||
120 μM | 4-hydroxybenzoate | |||||
90 μM | ATP | |||||
400 μM | CoA |
pH Dependence
Optimum pH is above 9.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4-hydroxybenzoate-CoA ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | benzoate-CoA ligase activity | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4-hydroxybenzoate--CoA/benzoate--CoA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Rhodopseudomonas
Accessions
- Primary accessionQ53005
- Secondary accessions
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_5000144018 | 1-539 | 4-hydroxybenzoate--CoA/benzoate--CoA ligase | ||
Post-translational modification
The N-terminus is blocked.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length539
- Mass (Da)58,930
- Last updated1996-11-01 v1
- MD5 Checksum21D4878FBF3FB3255282FEEC608143FB
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U02033 EMBL· GenBank· DDBJ | AAA62604.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX572595 EMBL· GenBank· DDBJ | CAE26113.1 EMBL· GenBank· DDBJ | Genomic DNA |