Q52K88 · NUD13_ARATH
- ProteinNudix hydrolase 13, mitochondrial
- GeneNUDT13
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids202 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use diadenosine 5',5'''-P1P6 hexaphosphate (Ap6A), diadenosine 5',5'''-P1P5 pentaphosphate (Ap5A) and adenosine tetraphosphate (p4A) as substrates, but not diadenosine 5',5'''-P1P4 tetraphosphate (Ap4A), diadenosine 5',5'''-P1P3 triphosphate (Ap3A), deoxyribonucleoside triphosphates, ribonucleoside triphosphates, diphosphoinositol pentakisphosphate (PP-InsP5) and 5-phospho-alpha-D-ribosyl diphosphate (PRPP).
Cofactor
Activity regulation
Inhibited by fluoride.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.61 mM | diadenosine 5',5'''-P1P6 hexaphosphate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
26.5 μmol/min/mg | with diadenosine 5',5'''-P1P6 hexaphosphate as substrate |
Reducing conditions are required for the hydrolytic activity.
pH Dependence
Optimum pH is 8.5.
Temperature Dependence
Optimum temperature is 37 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | bis(5'-adenosyl)-pentaphosphatase activity | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNudix hydrolase 13, mitochondrial
- EC number
- Short namesAtNUDT13
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ52K88
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 16 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, transit peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000019956 | ?-202 | Nudix hydrolase 13, mitochondrial | |||
Sequence: MSNLSARTGRDHQRYDNNFRLVSGCIPYRLVKDEEEDSTSVDFENKLQVLMISSPNRHDLVFPKGGWEDDETVLEAASREAMEEAGVKGILREDPLGVWEFRSKSSSVEADCCLGGGCKGYMFALEVKEELAIWPEQDDRERRWLNVKEALELCRYEWMQSALEEFLRVMAEEGSTKEDSLAISSISNRGERQIDPRYCFVV | ||||||
Transit peptide | 1-? | Mitochondrion |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, leaves, stems and inflorescences.
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 18-167 | Nudix hydrolase | ||||
Sequence: NFRLVSGCIPYRLVKDEEEDSTSVDFENKLQVLMISSPNRHDLVFPKGGWEDDETVLEAASREAMEEAGVKGILREDPLGVWEFRSKSSSVEADCCLGGGCKGYMFALEVKEELAIWPEQDDRERRWLNVKEALELCRYEWMQSALEEFL | ||||||
Motif | 65-86 | Nudix box | ||||
Sequence: GGWEDDETVLEAASREAMEEAG |
Sequence similarities
Belongs to the Nudix hydrolase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length202
- Mass (Da)23,187
- Last updated2005-05-24 v1
- ChecksumC8B20342322B8297
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB026648 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002686 EMBL· GenBank· DDBJ | AEE77199.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77200.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT021980 EMBL· GenBank· DDBJ | AAY17417.1 EMBL· GenBank· DDBJ | mRNA | ||
BT024767 EMBL· GenBank· DDBJ | ABD59105.1 EMBL· GenBank· DDBJ | mRNA |