Q52EB3 · ATG1_PYRO7
- ProteinSerine/threonine-protein kinase ATG1
- GeneATG1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids982 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Catalyzes phosphorylation of ATG4, decreasing the interaction between ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of ATG8 (By similarity).
Autophagy is essential to fungal development, production of appressorium turgor, and pathogenicity in rice blast disease (PubMed:17416896).
Autophagy is essential to fungal development, production of appressorium turgor, and pathogenicity in rice blast disease (PubMed:17416896).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Atg1/ULK1 kinase complex | |
Cellular Component | autophagosome membrane | |
Cellular Component | cytosol | |
Cellular Component | phagophore | |
Cellular Component | phagophore assembly site membrane | |
Cellular Component | vacuole-isolation membrane contact site | |
Molecular Function | ATP binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | appressorium formation | |
Biological Process | autophagic cell death | |
Biological Process | autophagosome assembly | |
Biological Process | conidium germination | |
Biological Process | piecemeal microautophagy of the nucleus | |
Biological Process | positive regulation of conidium formation | |
Biological Process | protein transport | |
Biological Process | regulation of autophagy | |
Biological Process | reticulophagy | |
Biological Process | spore germination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase ATG1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Magnaporthales > Pyriculariaceae > Pyricularia
Accessions
- Primary accessionQ52EB3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Preautophagosomal structure membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000085648 | 1-982 | Serine/threonine-protein kinase ATG1 | |||
Sequence: MADRSARRARPGDDSVGQFVIGAEIGKGSFAQVYMGKHKVSGAAVAIKSVELARLNKKLKENLYGEINILKTLRHPHIVALHDCVESATHINLMMEYCELGDLSLFIKKREKLSTNPATHDMARKYPNVPNSGLNEVVIRHFLKQLSSALKFLRESNLVHRDVKPQNLLLLPSPEFREVNKLARPILTASQDSLVPVAGLASLPMLKLADFGFARVLPSTSLAETLCGSPLYMAPEILRYERYDAKADLWSVGTVLFEMIVGRPPFRASNHVELLRKIEAAEDVIKFPRETTISSEMKGLTRALLKRNPVERISFENFFAHPVIISSIPGLVEDDIPKPEASEQRSSSKDTRAASKSDDPIASPRKYSFRRHPTDNDQIRDQFRRVEPPSSAAESAPSRQTSAFSGIAAEARKQAAAEASARTGQSSRNEPGDNLVPRRPQAQPSTSAPSKPGLYEERRRGISNASLNRSNRESSSPTSAALANDSARAPPQQTSRRVQAEEREKAAQDVAFERDYVVVEKKHVEVNAFADEMAANPRLGGQGTPLSPKSGQIVRRATQQGNPTSTTGAIPAAPSRTMQIAQGTQRHYHERGTSLSASPGSTASFITKAIQDASLRLLGIKYPAGLTKGASPPELYNPYPAYPTPSPPVGLISSGKQSTPVDEDARVAQLIEEHATRSDVVYGFAEVKYKQLVPLAPSAEHGLGGTTLEDMPTGEEDVLTVEAIVSLSEEALVLYVKALTLLAKSMDIASLWWARKNRGDQANNALSSTRDSVNTQTLSLRINSAVQWVRSRFNEVLEKAEVVRLRLMDAQKRLPDDHPSHPSNHPQGSESVNGASAEGVFLTVGVTAEKLMYDRALEMSRTAAINEITNEDLAGCEISYVTAIRMLEAVLDNDEDAPKRRLSANMDDSGGDGEDGHAEINADDQQAVQKMIHMIRSRLTSVRSKVRMISNASKAQQQSQPQSLIRRRSGDVTPRSVPSYSS |
Expression
Tissue specificity
Uniformly detected in conidia, mycelia and appressoria (at protein level).
Interaction
Subunit
Homodimer. Forms a ternary complex with ATG13 and ATG17.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-324 | Protein kinase | ||||
Sequence: FVIGAEIGKGSFAQVYMGKHKVSGAAVAIKSVELARLNKKLKENLYGEINILKTLRHPHIVALHDCVESATHINLMMEYCELGDLSLFIKKREKLSTNPATHDMARKYPNVPNSGLNEVVIRHFLKQLSSALKFLRESNLVHRDVKPQNLLLLPSPEFREVNKLARPILTASQDSLVPVAGLASLPMLKLADFGFARVLPSTSLAETLCGSPLYMAPEILRYERYDAKADLWSVGTVLFEMIVGRPPFRASNHVELLRKIEAAEDVIKFPRETTISSEMKGLTRALLKRNPVERISFENFFAHPVI | ||||||
Region | 334-506 | Disordered | ||||
Sequence: DDIPKPEASEQRSSSKDTRAASKSDDPIASPRKYSFRRHPTDNDQIRDQFRRVEPPSSAAESAPSRQTSAFSGIAAEARKQAAAEASARTGQSSRNEPGDNLVPRRPQAQPSTSAPSKPGLYEERRRGISNASLNRSNRESSSPTSAALANDSARAPPQQTSRRVQAEEREKA | ||||||
Compositional bias | 336-356 | Basic and acidic residues | ||||
Sequence: IPKPEASEQRSSSKDTRAASK | ||||||
Compositional bias | 363-384 | Basic and acidic residues | ||||
Sequence: SPRKYSFRRHPTDNDQIRDQFR | ||||||
Compositional bias | 393-407 | Polar residues | ||||
Sequence: AESAPSRQTSAFSGI | ||||||
Compositional bias | 464-496 | Polar residues | ||||
Sequence: NASLNRSNRESSSPTSAALANDSARAPPQQTSR | ||||||
Region | 813-834 | Disordered | ||||
Sequence: RLPDDHPSHPSNHPQGSESVNG | ||||||
Region | 898-918 | Disordered | ||||
Sequence: PKRRLSANMDDSGGDGEDGHA | ||||||
Region | 947-982 | Disordered | ||||
Sequence: RMISNASKAQQQSQPQSLIRRRSGDVTPRSVPSYSS |
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length982
- Mass (Da)107,443
- Last updated2005-06-07 v1
- Checksum9811E0BC78307638
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 336-356 | Basic and acidic residues | ||||
Sequence: IPKPEASEQRSSSKDTRAASK | ||||||
Compositional bias | 363-384 | Basic and acidic residues | ||||
Sequence: SPRKYSFRRHPTDNDQIRDQFR | ||||||
Compositional bias | 393-407 | Polar residues | ||||
Sequence: AESAPSRQTSAFSGI | ||||||
Compositional bias | 464-496 | Polar residues | ||||
Sequence: NASLNRSNRESSSPTSAALANDSARAPPQQTSR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ224381 EMBL· GenBank· DDBJ | ABB46201.1 EMBL· GenBank· DDBJ | mRNA | ||
CM001234 EMBL· GenBank· DDBJ | EHA50859.1 EMBL· GenBank· DDBJ | Genomic DNA |