Q51424 · RUVC_PSEAE
- ProteinCrossover junction endodeoxyribonuclease RuvC
- GeneruvC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids174 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
Complements an E.coli deletion mutant (PubMed:8982068).
A C-terminally truncated protein (residues 1-153) binds cruciform but not linear dsDNA, the consensus cleavage sequence is 5'-TT|C-3' (PubMed:32085896).
A C-terminally truncated protein (residues 1-153) binds cruciform but not linear dsDNA, the consensus cleavage sequence is 5'-TT|C-3' (PubMed:32085896).
Miscellaneous
Probably part of a ruvC-ruvA-ruvB operon. Does not seem to belong to the SOS regulon, no LexA binding site has been identified in the promoter region.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Mn2+ is a poor substitute for Mg2+ (PubMed:32085896).
Binds 2 Mg2+ ion per subunit.
Binds 2 Mg2+ ion per subunit.
Note: Binds 2 Mg2+ ion per subunit (By similarity).
Mn2+ is a poor substitute for Mg2+ (PubMed:32085896).
Mn2+ is a poor substitute for Mg2+ (PubMed:32085896).
pH Dependence
Optimum pH is 8.0 to 9.0.
Temperature Dependence
Optimum temperature is 37-55 degrees Celsius.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | Holliday junction resolvase complex | |
Molecular Function | crossover junction DNA endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrossover junction endodeoxyribonuclease RuvC
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ51424
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 8 | Does not cleave Holliday junction (HJ) DNA. | ||||
Sequence: D → N | ||||||
Mutagenesis | 12 | Does not cleave HJDNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 35 | Nearly wild-type cleavage of HJDNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 44 | Reduced cleavage of HJDNA, reduced binding to HJDNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 50 | Nearly wild-type cleavage of HJDNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 67 | Does not cleave HJDNA. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 70 | Does not cleave HJDNA, altered binding of HJDNA. | ||||
Sequence: F → A | ||||||
Mutagenesis | 73 | Does not cleave HJDNA, binds DNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 74 | Reduced cleavage of HJDNA. | ||||
Sequence: N → A | ||||||
Mutagenesis | 74 | Does not cleave HJDNA. | ||||
Sequence: N → K | ||||||
Mutagenesis | 139 | Does not cleave HJDNA. | ||||
Sequence: D → N | ||||||
Mutagenesis | 142 | Does not cleave HJDNA. | ||||
Sequence: D → N |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000183121 | 1-174 | Crossover junction endodeoxyribonuclease RuvC | |||
Sequence: MTLILGIDPGSRITGFGVVRETARGCEYVASGCIRTGNGPLHERLHVVFRSVREVIRTHGPTALSIEQVFMARNADSALKLGQARGAAIVAAMEEGLSVAEYTASQVKQAVVGTGGADKQQVQMMVMHLLKLTQKPQIDASDALAIALCHAHTQQSLVPHGLVGARRRGGRLRL |
Proteomic databases
Interaction
Subunit
Homodimer (PubMed:32085896).
Binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.
Binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length174
- Mass (Da)18,557
- Last updated1997-11-01 v1
- ChecksumE639B12CEF5FF517
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D83138 EMBL· GenBank· DDBJ | BAA11817.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE004091 EMBL· GenBank· DDBJ | AAG04354.1 EMBL· GenBank· DDBJ | Genomic DNA |