Q51424 · RUVC_PSEAE

Function

function

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
Complements an E.coli deletion mutant (PubMed:8982068).
A C-terminally truncated protein (residues 1-153) binds cruciform but not linear dsDNA, the consensus cleavage sequence is 5'-TT|C-3' (PubMed:32085896).

Miscellaneous

Probably part of a ruvC-ruvA-ruvB operon. Does not seem to belong to the SOS regulon, no LexA binding site has been identified in the promoter region.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Note: Mn2+ is a poor substitute for Mg2+ (PubMed:32085896).
Binds 2 Mg2+ ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ion per subunit (By similarity).
Mn2+ is a poor substitute for Mg2+ (PubMed:32085896).

pH Dependence

Optimum pH is 8.0 to 9.0.

Temperature Dependence

Optimum temperature is 37-55 degrees Celsius.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site8
Binding site8Mg2+ 1 (UniProtKB | ChEBI)
Active site67
Binding site67Mg2+ 2 (UniProtKB | ChEBI)
Active site139
Binding site139Mg2+ 1 (UniProtKB | ChEBI)
Active site142

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentHolliday junction resolvase complex
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease RuvC
  • EC number
  • Alternative names
    • Holliday junction nuclease RuvC
    • Holliday junction resolvase RuvC

Gene names

    • Name
      ruvC
    • Ordered locus names
      PA0965

Organism names

Accessions

  • Primary accession
    Q51424

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis8Does not cleave Holliday junction (HJ) DNA.
Mutagenesis12Does not cleave HJDNA.
Mutagenesis35Nearly wild-type cleavage of HJDNA.
Mutagenesis44Reduced cleavage of HJDNA, reduced binding to HJDNA.
Mutagenesis50Nearly wild-type cleavage of HJDNA.
Mutagenesis67Does not cleave HJDNA.
Mutagenesis70Does not cleave HJDNA, altered binding of HJDNA.
Mutagenesis73Does not cleave HJDNA, binds DNA.
Mutagenesis74Reduced cleavage of HJDNA.
Mutagenesis74Does not cleave HJDNA.
Mutagenesis139Does not cleave HJDNA.
Mutagenesis142Does not cleave HJDNA.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001831211-174Crossover junction endodeoxyribonuclease RuvC

Proteomic databases

Interaction

Subunit

Homodimer (PubMed:32085896).
Binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the RuvC family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    174
  • Mass (Da)
    18,557
  • Last updated
    1997-11-01 v1
  • Checksum
    E639B12CEF5FF517
MTLILGIDPGSRITGFGVVRETARGCEYVASGCIRTGNGPLHERLHVVFRSVREVIRTHGPTALSIEQVFMARNADSALKLGQARGAAIVAAMEEGLSVAEYTASQVKQAVVGTGGADKQQVQMMVMHLLKLTQKPQIDASDALAIALCHAHTQQSLVPHGLVGARRRGGRLRL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D83138
EMBL· GenBank· DDBJ
BAA11817.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004091
EMBL· GenBank· DDBJ
AAG04354.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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