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Q50HP4 · DAD4_SCHPO

Function

function

Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase (PubMed:16079914, PubMed:20624975).
Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules (PubMed:16079914, PubMed:20624975).
Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle (PubMed:16079914).
The DASH complex mediates bipolar kinetochore-microtubule attachments and facilitates the formation of additional interactions between outer kinetochore components and spindle microtubules (PubMed:16079914).
During chromosome movement along the microtubule, it is required both for the sliding of kinetochores along the lateral side of the microtubule and also for microtubule end-on pulling on the kinetochore (PubMed:18256284).
Modulates cytoplasmic microtubule dynamics by tracking the plus-end of shortening microtubules and slowing their depolymerization (PubMed:20624975).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular ComponentDASH complex
Cellular Componentmicrotubule
Cellular Componentmitotic spindle
Biological Processattachment of spindle microtubules to kinetochore
Biological Processcell division
Biological Processmitotic sister chromatid biorientation
Biological Processprotein transport along microtubule to mitotic spindle pole body
Biological Processspindle attachment to meiosis I kinetochore

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    DASH complex subunit dad4
  • Alternative names
    • Outer kinetochore protein dad4

Gene names

    • Name
      dad4
    • ORF names
      SPBC3B9.22c

Organism names

Accessions

  • Primary accession
    Q50HP4

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Note: Associates with the mitotic spindle and the kinetochore. Kinetochore association occurs only during mitosis (By similarity).
In the cytoskeleton, localizes to cortical microtubules (Probable)

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001760511-72DASH complex subunit dad4

Proteomic databases

PTM databases

Interaction

Subunit

Component of the DASH complex consisting of ask1, dad1, dad2, dad3, dad4, dam1, duo1, dad5, spc19 and spc34, with a stoichiometry of one copy of each subunit per complex (PubMed:16079914).
Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore (By similarity).
DASH complex oligomerization strengthens microtubule attachments (By similarity).
On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings (PubMed:20624975).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for coiled coil.

TypeIDPosition(s)Description
Coiled coil15-35

Sequence similarities

Belongs to the DASH complex DAD4 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    72
  • Mass (Da)
    8,351
  • Last updated
    2006-05-16 v2
  • MD5 Checksum
    0A874A0180CB32967190CBBC1E18CB61
MNNPMEEQQSALLGRIISNVEKLNESITRLNHSLQLINMSNMNVELASQMWANYARNVKFHLEETHTLKDPI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CU329671
EMBL· GenBank· DDBJ
CAI94506.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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