Q50746 · MOAA_METTM
- ProteinProbable GTP 3',8-cyclase
- GenemoaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids305 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity
- AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H+ + L-methionine
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 22 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 26 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 28 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 29 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 62 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 66 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 92 | GTP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 116 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 153 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 249 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 252 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 254-256 | GTP (UniProtKB | ChEBI) | ||||
Sequence: RLR | ||||||
Binding site | 266 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cyclic pyranopterin monophosphate synthase activity | |
Molecular Function | GTP 3',8'-cyclase activity | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable GTP 3',8-cyclase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionQ50746
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000153021 | 1-305 | Probable GTP 3',8-cyclase | |||
Sequence: MQVHDNHRRPLVSLRISVTGRCNVSCIYCHRDGILRSDEEMSPEDIENICRVASDLGVKKIRLSGGEPLIRDDIVEIVEKINSIGFRDISITTNGTLLEDLSVPLRDAGLDRVNVSFDTLKPETYRFITRKDYLERVKAGIEGAVMAGLDPVKINMVILRGVNHHEIWDMFEFCRQQGAVLQIIELLKTDSCPDNGVERYHCDITPIEAELAEMADRIMTRKFMQDRKKYFIGDGEVEVVRPMDNTRFCANCTRLRVTPDGKLKPCLLRNDNLVDTKEALSSGDLEGLRELFLEAIRRRSPYYQS |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-228 | Radical SAM core | ||||
Sequence: NHRRPLVSLRISVTGRCNVSCIYCHRDGILRSDEEMSPEDIENICRVASDLGVKKIRLSGGEPLIRDDIVEIVEKINSIGFRDISITTNGTLLEDLSVPLRDAGLDRVNVSFDTLKPETYRFITRKDYLERVKAGIEGAVMAGLDPVKINMVILRGVNHHEIWDMFEFCRQQGAVLQIIELLKTDSCPDNGVERYHCDITPIEAELAEMADRIMTRKFMQDRK |
Sequence similarities
Belongs to the radical SAM superfamily. MoaA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)34,831
- Last updated2010-11-30 v2
- Checksum36B65CED21C1D260
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X87968 EMBL· GenBank· DDBJ | CAA61207.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001710 EMBL· GenBank· DDBJ | ADL57745.1 EMBL· GenBank· DDBJ | Genomic DNA |