Q4WRY5 · LAEA_ASPFU
- ProteinSecondary metabolism regulator laeA
- GenelaeA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids373 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Methyltransferase that performs automethylation (By similarity).
No other methyl-accepting substrate has been identified yet (By similarity).
Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:15075281, PubMed:17630330, PubMed:19843884, PubMed:24082142, PubMed:24116213).
Positively controls expression of 20% to 40% of major classes of secondary metabolite biosynthesis genes such as nonribosomal peptide synthetases, polyketide synthases, and P450 monooxygenases (PubMed:17432932).
Controls the expression of the gliotoxin gene cluster (PubMed:15075281, PubMed:17630330, PubMed:19843884).
Controls the expression of the fumitremorgin, fumagillin, and pseurotin gene clusters, where genes for fumagillin and pseurotin are physically intertwined in a single supercluster (PubMed:24082142).
Regulates the biosynthetic genes required for endocrocin production (PubMed:22492455).
Secondary metabolites under the transcriptional regulation of laeA are necessary for inhibition of angiogenesis during invasive infection in mice (PubMed:19843884).
Controls the expression of cell surface rodA, a hydrophobin that acts as an antiphagocytic molecule (PubMed:19917717).
Regulates also the expression of genes involved in conidial biosynthesis (PubMed:16151250).
No other methyl-accepting substrate has been identified yet (By similarity).
Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:15075281, PubMed:17630330, PubMed:19843884, PubMed:24082142, PubMed:24116213).
Positively controls expression of 20% to 40% of major classes of secondary metabolite biosynthesis genes such as nonribosomal peptide synthetases, polyketide synthases, and P450 monooxygenases (PubMed:17432932).
Controls the expression of the gliotoxin gene cluster (PubMed:15075281, PubMed:17630330, PubMed:19843884).
Controls the expression of the fumitremorgin, fumagillin, and pseurotin gene clusters, where genes for fumagillin and pseurotin are physically intertwined in a single supercluster (PubMed:24082142).
Regulates the biosynthetic genes required for endocrocin production (PubMed:22492455).
Secondary metabolites under the transcriptional regulation of laeA are necessary for inhibition of angiogenesis during invasive infection in mice (PubMed:19843884).
Controls the expression of cell surface rodA, a hydrophobin that acts as an antiphagocytic molecule (PubMed:19917717).
Regulates also the expression of genes involved in conidial biosynthesis (PubMed:16151250).
Catalytic activity
- L-methionyl-[protein] + S-adenosyl-L-methionine = S-methyl-L-methionyl-[protein] + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | methyltransferase activity | |
Biological Process | methylation | |
Biological Process | positive regulation of gliotoxin biosynthetic process | |
Biological Process | regulation of secondary metabolic process | |
Biological Process | regulation of secondary metabolite biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process | |
Biological Process | sporulation resulting in formation of a cellular spore | |
Biological Process | symbiont-mediated perturbation of host phagocytosis |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSecondary metabolism regulator laeA
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Fumigati
Accessions
- Primary accessionQ4WRY5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Reduces the production of the immunotoxin gliotoxin (PubMed:15075281, PubMed:16151250, PubMed:17630330).
Reduces the production of endocrocin (PubMed:22492455).
Affects transcription of numerous secondary metabolite biosynthesis genes (PubMed:17432932).
Decreases spore formation and germination (PubMed:23022264).
Reduces virulence in a murine pulmonary infection model through increased phagocytosis by host macrophages (PubMed:16151250, PubMed:19917717).
Reduces the production of endocrocin (PubMed:22492455).
Affects transcription of numerous secondary metabolite biosynthesis genes (PubMed:17432932).
Decreases spore formation and germination (PubMed:23022264).
Reduces virulence in a murine pulmonary infection model through increased phagocytosis by host macrophages (PubMed:16151250, PubMed:19917717).
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000435743 | 1-373 | Secondary metabolism regulator laeA | |||
Sequence: MFLNGQGGQRPPMVAFPPLNVRGSISSGFNALGRSRNNSDAMDIYTITDRGPAAERDPAAGRWHANGSPSINSTSSKNPDRYPCYQENGRTYHGYRKGIYMLPCDEQEQDRLDIFHKLFTVARVSDGLIYAPHPTNGRFLDLGCGTGIWAIDVANKYPEAFVVGVDLAPIQPPNHPRNCDFYAPFDFESLWALGEDSWDLIHMQMGSGSVASWPNLYRRIYSHLRPGAWFEQVEIDFEPRCDDRSLEGLAIRQWYQLLKQATEETMRPVAHNSRETIRNLQEAGFTEIDHQMVGLPLNPWHEDEHERRVARWYNLAISESIETMSLAPFSRVFGWPIERIKQIAADVKSEAFNKEIHTYNILHIYQARKPLAN |
Expression
Induction
Expression is induces in response to antifungal activity produced by Lactobacillus plantarum (PubMed:23876797).
Interaction
Subunit
Component of the heterotrimeric velvet complex composed of laeA, veA and velB; VeA acting as a bridging protein between laeA and velB (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 55-81 | Disordered | ||||
Sequence: ERDPAAGRWHANGSPSINSTSSKNPDR | ||||||
Compositional bias | 66-81 | Polar residues | ||||
Sequence: NGSPSINSTSSKNPDR |
Sequence similarities
Belongs to the methyltransferase superfamily. LaeA methyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length373
- Mass (Da)42,581
- Last updated2005-07-05 v1
- Checksum193DF67DB76FB644
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 66-81 | Polar residues | ||||
Sequence: NGSPSINSTSSKNPDR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY422723 EMBL· GenBank· DDBJ | AAR01218.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AAHF01000004 EMBL· GenBank· DDBJ | EAL90797.1 EMBL· GenBank· DDBJ | Genomic DNA |