Q4WRY5 · LAEA_ASPFU

Function

function

Methyltransferase that performs automethylation (By similarity).
No other methyl-accepting substrate has been identified yet (By similarity).
Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:15075281, PubMed:17630330, PubMed:19843884, PubMed:24082142, PubMed:24116213).
Positively controls expression of 20% to 40% of major classes of secondary metabolite biosynthesis genes such as nonribosomal peptide synthetases, polyketide synthases, and P450 monooxygenases (PubMed:17432932).
Controls the expression of the gliotoxin gene cluster (PubMed:15075281, PubMed:17630330, PubMed:19843884).
Controls the expression of the fumitremorgin, fumagillin, and pseurotin gene clusters, where genes for fumagillin and pseurotin are physically intertwined in a single supercluster (PubMed:24082142).
Regulates the biosynthetic genes required for endocrocin production (PubMed:22492455).
Secondary metabolites under the transcriptional regulation of laeA are necessary for inhibition of angiogenesis during invasive infection in mice (PubMed:19843884).
Controls the expression of cell surface rodA, a hydrophobin that acts as an antiphagocytic molecule (PubMed:19917717).
Regulates also the expression of genes involved in conidial biosynthesis (PubMed:16151250).

Catalytic activity

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular Functionmethyltransferase activity
Biological Processmethylation
Biological Processpositive regulation of gliotoxin biosynthetic process
Biological Processregulation of secondary metabolic process
Biological Processregulation of secondary metabolite biosynthetic process
Biological Processsecondary metabolite biosynthetic process
Biological Processsporulation resulting in formation of a cellular spore
Biological Processsymbiont-mediated perturbation of host phagocytosis

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Secondary metabolism regulator laeA
  • Alternative names
    • Methyltransferase laeA
      (EC:2.1.1.-
      ) . EC:2.1.1.- (UniProtKB | ENZYME | Rhea)
    • Velvet complex subunit laeA

Gene names

    • Name
      laeA
    • ORF names
      AFUA_1G14660

Organism names

Accessions

  • Primary accession
    Q4WRY5
  • Secondary accessions
    • Q6TFC7

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Reduces the production of the immunotoxin gliotoxin (PubMed:15075281, PubMed:16151250, PubMed:17630330).
Reduces the production of endocrocin (PubMed:22492455).
Affects transcription of numerous secondary metabolite biosynthesis genes (PubMed:17432932).
Decreases spore formation and germination (PubMed:23022264).
Reduces virulence in a murine pulmonary infection model through increased phagocytosis by host macrophages (PubMed:16151250, PubMed:19917717).

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004357431-373Secondary metabolism regulator laeA

Expression

Induction

Expression is induces in response to antifungal activity produced by Lactobacillus plantarum (PubMed:23876797).

Interaction

Subunit

Component of the heterotrimeric velvet complex composed of laeA, veA and velB; VeA acting as a bridging protein between laeA and velB (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region55-81Disordered
Compositional bias66-81Polar residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    373
  • Mass (Da)
    42,581
  • Last updated
    2005-07-05 v1
  • Checksum
    193DF67DB76FB644
MFLNGQGGQRPPMVAFPPLNVRGSISSGFNALGRSRNNSDAMDIYTITDRGPAAERDPAAGRWHANGSPSINSTSSKNPDRYPCYQENGRTYHGYRKGIYMLPCDEQEQDRLDIFHKLFTVARVSDGLIYAPHPTNGRFLDLGCGTGIWAIDVANKYPEAFVVGVDLAPIQPPNHPRNCDFYAPFDFESLWALGEDSWDLIHMQMGSGSVASWPNLYRRIYSHLRPGAWFEQVEIDFEPRCDDRSLEGLAIRQWYQLLKQATEETMRPVAHNSRETIRNLQEAGFTEIDHQMVGLPLNPWHEDEHERRVARWYNLAISESIETMSLAPFSRVFGWPIERIKQIAADVKSEAFNKEIHTYNILHIYQARKPLAN

Sequence caution

The sequence AAR01218.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias66-81Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY422723
EMBL· GenBank· DDBJ
AAR01218.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AAHF01000004
EMBL· GenBank· DDBJ
EAL90797.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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