Q4WQY6 · TPCL_ASPFU

Function

function

Anthrone oxygenase; part of the gene cluster that mediates the biosynthesis of trypacidin, a mycotoxin with antiprotozoal activity and that plays a role in the infection process (PubMed:26242966, PubMed:26278536).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) tpcC (PubMed:26242966).
The atrochrysone carboxyl ACP thioesterase tpcB then breaks the thioester bond and releases the atrochrysone carboxylic acid from tpcC (PubMed:26242966).
The decarboxylase tpcK converts atrochrysone carboxylic acid to atrochrysone which is further reduced into emodin anthrone (PubMed:26242966).
The next step is performed by the emodin anthrone oxygenase tpcL that catalyzes the oxidation of emodinanthrone to emodin (PubMed:26242966).
Emodin O-methyltransferase encoded by tpcA catalyzes methylation of the 8-hydroxy group of emodin to form questin (PubMed:26242966).
Ring cleavage of questin by questin oxidase tpcI leads to desmethylsulochrin via several intermediates including questin epoxide (By similarity).
Another methylation step catalyzed by tpcM leads to the formation of sulochrin which is further converted to monomethylsulfochrin by tpcH. Finally, the tpcJ catalyzes the conversion of monomethylsulfochrin to trypacidin (PubMed:26242966).
Trypacidin is toxic for human pulmonary and bronchial epithelial cells by initiating the intracellular formation of nitric oxide (NO) and hydrogen peroxide (H2O2), thus triggering host necrotic cell death (PubMed:22319557).
The trypacidin pathway is also able to produce endocrocin via a distinct route from the endocrocin Enc pathway (PubMed:26242966).

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionmonooxygenase activity
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Anthrone oxygenase tpcL
  • EC number
  • Alternative names
    • Trypacidin synthesis protein L

Gene names

    • Name
      tpcL
    • Synonyms
      tynL
    • ORF names
      AFUA_4G14480

Organism names

Accessions

  • Primary accession
    Q4WQY6

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane15-35Helical
Transmembrane56-74Helical
Transmembrane87-107Helical
Transmembrane136-155Helical

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004370981-161Anthrone oxygenase tpcL
Glycosylation4N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Tissue specificity

Specifically expressed in conidia (PubMed:22319557).

Induction

Expression is positively regulated by the transcription factors brlA and laeA (PubMed:26242966).

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the anthrone oxygenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    161
  • Mass (Da)
    17,361
  • Last updated
    2007-04-17 v2
  • Checksum
    4EF73E3BB7043B9E
MPQNASVIITQATAVITGSFLSGLMMGLSVVDIPVVLDTATQASQLLQHFTRLYDIGHKMMPSLAVTTCLLYGYTASSTRTTGGSGLPHIIAAVTTISMVPFTWLVMAPTNNALFRMHANPAAANLGEVRRLLVRWAQLHAVRSLFPLMGSVLGLRQILRE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHF01000005
EMBL· GenBank· DDBJ
EAL89348.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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