Q4WQM4 · HAS1_ASPFU
- ProteinATP-dependent RNA helicase has1
- Genehas1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids622 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nuclear envelope | |
Cellular Component | nucleolus | |
Cellular Component | preribosome, large subunit precursor | |
Cellular Component | small-subunit processome | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | identical protein binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) | |
Biological Process | maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) | |
Biological Process | snoRNA release from pre-rRNA |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent RNA helicase has1
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Fumigati
Accessions
- Primary accessionQ4WQM4
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000232205 | 1-622 | ATP-dependent RNA helicase has1 | |||
Sequence: MSGPVDTAKSITKKRKRKHGGGARAATETDDAITRPAIENGAVNDSPEKEEDTKKSEKNGKDKSAKKRKVSHASSDEGDESQEEQGAPAQADGDSDDNKDDGNDQSEAENGDNGDKKDTESTDLPSAGTLSLPTVEGEPQKFTELGLSEKTLKAINDMGFETMTEIQRRTIPPLLAGRDVLGAAKTGSGKTLSFLIPAVEMLSALRFKPRNGTGVIVVSPTRELALQIFGVARELCQYHSQTYGIVIGGANRRAEAEKLMKGVNLLIATPGRLLDHLQNTQGFVFKNLKTLVIDEADRILEVGFEDEMRQIVKILPSEERQTMLFSATQTTKVEDLARISLRPGPLYINVDHRKEHSTVEGLEQGYVICEADKRFLLLFSFLKRNLKKKIIVFFSSCNCVKYHAELLNYIDLPVLELHGKQKQQKRTNTFFEFCNAKQGTLICTDVAARGLDIPAVDWIIQFDPPDDPRDYIHRVGRTARGTNAKGRSLMFLQPSEVGFLKHLKEARVPVVEFEFPANKIVNVQSQLEKLIGQNYYLNKSAKEGYRSYLQAYASHSLRSVFDVHKLDLVKVAKGFGFSTPPRIDIQLGASLSRDKKQQQQGRRSYGSQPHSKGLKFKRKHDD |
Interaction
Subunit
Associates in the nucleolus with the 60S and pre-60S ribosomal subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-143 | Disordered | ||||
Sequence: MSGPVDTAKSITKKRKRKHGGGARAATETDDAITRPAIENGAVNDSPEKEEDTKKSEKNGKDKSAKKRKVSHASSDEGDESQEEQGAPAQADGDSDDNKDDGNDQSEAENGDNGDKKDTESTDLPSAGTLSLPTVEGEPQKFT | ||||||
Compositional bias | 44-79 | Basic and acidic residues | ||||
Sequence: NDSPEKEEDTKKSEKNGKDKSAKKRKVSHASSDEGD | ||||||
Compositional bias | 104-122 | Basic and acidic residues | ||||
Sequence: DQSEAENGDNGDKKDTEST | ||||||
Motif | 140-168 | Q motif | ||||
Sequence: QKFTELGLSEKTLKAINDMGFETMTEIQR | ||||||
Domain | 171-347 | Helicase ATP-binding | ||||
Sequence: IPPLLAGRDVLGAAKTGSGKTLSFLIPAVEMLSALRFKPRNGTGVIVVSPTRELALQIFGVARELCQYHSQTYGIVIGGANRRAEAEKLMKGVNLLIATPGRLLDHLQNTQGFVFKNLKTLVIDEADRILEVGFEDEMRQIVKILPSEERQTMLFSATQTTKVEDLARISLRPGPLY | ||||||
Motif | 294-297 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 361-531 | Helicase C-terminal | ||||
Sequence: GLEQGYVICEADKRFLLLFSFLKRNLKKKIIVFFSSCNCVKYHAELLNYIDLPVLELHGKQKQQKRTNTFFEFCNAKQGTLICTDVAARGLDIPAVDWIIQFDPPDDPRDYIHRVGRTARGTNAKGRSLMFLQPSEVGFLKHLKEARVPVVEFEFPANKIVNVQSQLEKLI | ||||||
Motif | 373-389 | Bipartite nuclear localization signal | ||||
Sequence: KRFLLLFSFLKRNLKKK | ||||||
Region | 588-622 | Disordered | ||||
Sequence: GASLSRDKKQQQQGRRSYGSQPHSKGLKFKRKHDD | ||||||
Compositional bias | 591-607 | Polar residues | ||||
Sequence: LSRDKKQQQQGRRSYGS |
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length622
- Mass (Da)69,202
- Last updated2006-04-04 v2
- Checksum4C2226E7F937CE74
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 44-79 | Basic and acidic residues | ||||
Sequence: NDSPEKEEDTKKSEKNGKDKSAKKRKVSHASSDEGD | ||||||
Compositional bias | 104-122 | Basic and acidic residues | ||||
Sequence: DQSEAENGDNGDKKDTEST | ||||||
Compositional bias | 591-607 | Polar residues | ||||
Sequence: LSRDKKQQQQGRRSYGS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHF01000005 EMBL· GenBank· DDBJ | EAL89460.2 EMBL· GenBank· DDBJ | Genomic DNA |