Q4WM22 · SITA_ASPFU

Function

function

Protein phosphatase that acts as a modulator of pkcA/mpkA activity involved in the cell wall integrity pathway (PubMed:25911225).
Plays an important role in regulation of adhesion, cell wall integrity, biofilm formation, and virulence (PubMed:25911225).

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site67Mn2+ 1 (UniProtKB | ChEBI)
Binding site69Mn2+ 1 (UniProtKB | ChEBI)
Binding site161Mn2+ 1 (UniProtKB | ChEBI)
Binding site161Mn2+ 2 (UniProtKB | ChEBI)
Binding site193Mn2+ 2 (UniProtKB | ChEBI)
Active site194Proton donor
Binding site243Mn2+ 2 (UniProtKB | ChEBI)
Binding site317Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchromatin
Cellular Componentcytoplasm
Cellular Componentprotein phosphatase type 2A complex
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionprotein serine/threonine phosphatase activity
Biological Processcell adhesion involved in biofilm formation
Biological Processcellular response to oxidative stress
Biological ProcessDNA repair
Biological Processfungal-type cell wall organization
Biological ProcessG1/S transition of mitotic cell cycle
Biological Processintracellular signal transduction
Biological Processnegative regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway
Biological Processregulation of actin cytoskeleton organization
Biological Processregulation of fungal-type cell wall organization
Biological Processregulation of TORC1 signaling
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein phosphatase sitA
  • EC number

Gene names

    • Name
      sitA
    • ORF names
      AFUA_6G11470

Organism names

Accessions

  • Primary accession
    Q4WM22

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Leads to decreased mpkA phosphorylation levels, increased sensitivity to cell wall-damaging agents, increased levels of beta-(1,3)-glucan and chitin, impaired in biofilm formation, and decreased protein kinase C activity (PubMed:25911225).
Increases also sensitivity to several metals and ions, such as MnCl2, CaCl2, and LiCl, but it is more resistant to ZnSO4 (PubMed:25911225).
Impeairs the virulencein a murine model of invasive pulmonary aspergillosis and induces an augmented tumor necrosis factor alpha response in mouse macrophages (PubMed:25911225).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004531821-388Serine/threonine-protein phosphatase sitA

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region86-146Disordered
Compositional bias95-109Polar residues
Compositional bias111-127Basic and acidic residues
Compositional bias128-143Polar residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    388
  • Mass (Da)
    43,535
  • Last updated
    2007-04-17 v2
  • Checksum
    931FB3CFA3E16037
MVDNKIPEPGPAKLKRNAGPDEWLEAAKDCKYLSEQHMKQLCEIVKEYMMEESNIQPVSTPVTICGDIHGQFYDLLELFRVSGGMPDGSEAEAPKTQSAVITSDDIEPPSTISDPKLRKKLRNTFAPEDGSEDSSASQRDRSSSSGSRDVELKRNFVFLGDYVDRGYFSLETLTLLLCLKAKYPDRVTLVRGNHESRQITQVYGFYEECFQKYGNASVWKACCQVFDFMTLGAIIDGRVLCVHGGLSPEIRTLDQVRVVARAQEIPHEGAFCDLVWSDPDDVETWAVSPRGAGWLFGDKVADEFCHVNDLTLIARAHQLVNEGYKYHFANQNVVTVWSAPNYCYRCGNLASVCEIGDDLKPTFKLFSAVSDDQRHVPVSRPGRSEYFL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias95-109Polar residues
Compositional bias111-127Basic and acidic residues
Compositional bias128-143Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHF01000006
EMBL· GenBank· DDBJ
EAL88992.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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