Q4VYA5 · HIUH_SALDU
- Protein5-hydroxyisourate hydrolase
- GenehiuH
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids136 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).
Miscellaneous
Despite its homology to transthyretins, is not able to bind to the thyroid hormones thyroxine (T4) and triiodothyronine (T3).
HIU hydrolysis also occurs spontaneously, but more slowly.
Catalytic activity
- 5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 31 | substrate | ||||
Sequence: H | ||||||
Binding site | 69 | substrate | ||||
Sequence: R | ||||||
Binding site | 133 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | hydroxyisourate hydrolase activity | |
Biological Process | purine nucleobase metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5-hydroxyisourate hydrolase
- EC number
- Short namesHIU hydrolase; HIUHase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionQ4VYA5
- Secondary accessions
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 31 | Loss of activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 120 | Reduces activity by 90%. | ||||
Sequence: H → A | ||||||
Mutagenesis | 133 | Reduces activity by 90%. | ||||
Sequence: Y → F |
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MKRHILATVIASLVAAPAMALA | ||||||
Chain | PRO_5000074934 | 23-136 | 5-hydroxyisourate hydrolase | |||
Sequence: AGNNILSVHILDQQTGKPAPGVEVVLEQKKDNGWTQLNTGHTDQDGRIKALWPEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHISKTNEHYHVPLLLSQYGYSTYRGS |
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length136
- Mass (Da)15,105
- Last updated2005-07-05 v1
- Checksum6515448D6B1F889D
Sequence caution
Mass Spectrometry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF060858 EMBL· GenBank· DDBJ | AAC33718.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
AJ972893 EMBL· GenBank· DDBJ | CAI99863.1 EMBL· GenBank· DDBJ | Genomic DNA |