Q4UIR6 · Q4UIR6_THEAN
- ProteinQueuine tRNA-ribosyltransferase catalytic subunit 1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids407 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.
Catalytic activity
- guanosine34 in tRNA + queuine = queuosine34 in tRNA + guanine
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 91 | Proton acceptor | |||
Binding site | 91-95 | substrate | |||
Binding site | 146 | substrate | |||
Binding site | 190 | substrate | |||
Binding site | 217 | substrate | |||
Active site | 270 | Nucleophile | |||
Binding site | 308 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 310 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 313 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 338 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-guanosine(34) queuine transglycosylase activity | |
Biological Process | tRNA-guanine transglycosylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQueuine tRNA-ribosyltransferase catalytic subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Piroplasmida > Theileriidae > Theileria
Accessions
- Primary accessionQ4UIR6
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Heterodimer of a catalytic subunit and an accessory subunit.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 13-370 | tRNA-guanine15 transglycosylase-like | |||
Region | 251-257 | RNA binding | |||
Region | 275-279 | RNA binding; important for wobble base 34 recognition | |||
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length407
- Mass (Da)45,860
- Last updated2005-07-05 v1
- MD5 ChecksumA534D9B8B76D37BB419F00DE0CD7CD9C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR940347 EMBL· GenBank· DDBJ | CAI73023.1 EMBL· GenBank· DDBJ | Genomic DNA |