Q4UIR6 · Q4UIR6_THEAN

  • Protein
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site91Proton acceptor
Binding site91-95substrate
Binding site146substrate
Binding site190substrate
Binding site217substrate
Active site270Nucleophile
Binding site308Zn2+ (UniProtKB | ChEBI)
Binding site310Zn2+ (UniProtKB | ChEBI)
Binding site313Zn2+ (UniProtKB | ChEBI)
Binding site338Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • ORF names
      TA16175

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Ankara
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Piroplasmida > Theileriidae > Theileria

Accessions

  • Primary accession
    Q4UIR6

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Interaction

Subunit

Heterodimer of a catalytic subunit and an accessory subunit.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain13-370tRNA-guanine15 transglycosylase-like
Region251-257RNA binding
Region275-279RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    407
  • Mass (Da)
    45,860
  • Last updated
    2005-07-05 v1
  • MD5 Checksum
    A534D9B8B76D37BB419F00DE0CD7CD9C
MALKFNVVASDGRARRGELVLTHGLVETPVFMPVGTKGAIKGLCYDQLESLGCVLSLGNTFHLAFHPGSEIIRKGGGLHKFTNSRCNFLTDSGGFQMVSLFSLSRVTEEGVEFKFPYDLQREIFLTPEESIKIQNDIGADIIMALDDVVSSLVRDKERLEVATHRTTRWLDRCIKAHSNPETQALFAIVQGSIYPDLRIISLNELKKRDLPGYAIGGLSGGENKTDFWRIVELCTREGVGLPSNKPRYLMGVGYQIDLIVSVALGCDMFDCVFPTRTARFGTALNDHGNIRIQHEIYQYDFSPIDQDCDCLCCLKYSRAFLHYGLKKTILVSHLLTLHNIRYMVRLFERIRKSISSGNFKGFVRRFLTNMYINNGELPPLWVRDSLLSAGIDILDLYPDNSNVVKPY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR940347
EMBL· GenBank· DDBJ
CAI73023.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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