Q4U0Y4 · NPL1A_XENLA
- ProteinNucleosome assembly protein 1-like 1-A
- Genenap1l1-a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids392 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a chaperone for the linker histone to facilitate deposition of histone B4 onto linker DNA (PubMed:14550533, PubMed:15811954, PubMed:15928086, PubMed:16982648).
Required for both remodeling of sperm chromatin into nucleosomes, and linker histone binding to nucleosome core dimers. Plays a role in tissue-specific gene regulation. Required for primitive hemopoiesis, acting upstream of tal1/scl (PubMed:14550533, PubMed:15811954, PubMed:15928086, PubMed:16982648).
Required for both remodeling of sperm chromatin into nucleosomes, and linker histone binding to nucleosome core dimers. Plays a role in tissue-specific gene regulation. Required for primitive hemopoiesis, acting upstream of tal1/scl (PubMed:14550533, PubMed:15811954, PubMed:15928086, PubMed:16982648).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromatin | |
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | chromatin binding | |
Molecular Function | cyclin binding | |
Molecular Function | histone binding | |
Molecular Function | identical protein binding | |
Biological Process | nucleosome assembly | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | primitive hemopoiesis | |
Biological Process | spermatogenesis |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNucleosome assembly protein 1-like 1-A
- Short namesxNAP1L-A
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ4U0Y4
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000345634 | 1-392 | Nucleosome assembly protein 1-like 1-A | |||
Sequence: MANIDNKGQTELDQQDMEDVEDVEEEETGEDANSKARQLTAQMMQNPQVLAALQERLDDLVGTPTGYIESLPKVVKRRVNALKNLQVKCAQIEAKFYEEVHELERKYAALYQPLFDKRSDIINATYEPTEEECEWKVEEEDISGDLKEKAKLEEEKKDEEKEDPKGIPEFWLTVFKNVDLLSDMLQEHDEPILKHLKDIKVKFSDAGQPMSFTLEFYFEPNEFFTNEVLTKTYKMRSEPDESDPFSFDGPEIMGCTGCLIDWKKGKNVTLKTIKKKQKHKGRGTVRTVTKTVPNDSFFNFFTPPEVPENGELDDDAEAILTADFEIGHFLRERIIPRSVLYFTGEAIEDDDDDYDEEGEEADDEEGEEEADEDNDPDYEPKKGQNPAECKQQ |
Post-translational modification
Phosphorylated by cyclin B-cdc2 kinase complexes.
Keywords
- PTM
Expression
Tissue specificity
Initially expressed throughout the embryo with expression higher at the animal pole. Becomes localized to presumptive ectoderm by gastrula stages. By stage 18 (neurula), expressed in the neural plate and posterior to the cement gland. In late neurula/early tailbud stages, expressed in the neural crest, neural tube, eyes, tailbud and ventral blood islands. Adult expression is predominantly in ovaries.
Developmental stage
Expressed both maternally and zygotically. Expression levels remain constant during embryonic development, increasing at the swimming tadpole stage.
Gene expression databases
Interaction
Subunit
Forms homomultimers. Interacts with histone B4. Interacts with the B-type cyclins ccnb1 and ccnb2.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-37 | Disordered | ||||
Sequence: MANIDNKGQTELDQQDMEDVEDVEEEETGEDANSKAR | ||||||
Compositional bias | 15-29 | Acidic residues | ||||
Sequence: QDMEDVEDVEEEETG | ||||||
Motif | 126-150 | NAP1L motif | ||||
Sequence: YEPTEEECEWKVEEEDISGDLKEKA | ||||||
Motif | 273-279 | Nuclear localization signal | ||||
Sequence: IKKKQKH | ||||||
Compositional bias | 346-375 | Acidic residues | ||||
Sequence: AIEDDDDDYDEEGEEADDEEGEEEADEDND | ||||||
Region | 346-392 | Disordered | ||||
Sequence: AIEDDDDDYDEEGEEADDEEGEEEADEDNDPDYEPKKGQNPAECKQQ |
Domain
The NAP1L motif is required for the histone chaperone activity.
The acidic domains are probably involved in the interaction with histones.
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q4U0Y4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Synonymsp60A
- Length392
- Mass (Da)45,187
- Last updated2005-07-19 v1
- Checksum74960F50F2BA0E78
Q4U0Y4-2
- Name2
- Synonymsp56A
- Differences from canonical
- 382-392: KGQNPAECKQQ → V
Sequence caution
Features
Showing features for sequence conflict, compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 3 | in Ref. 1 | ||||
Sequence: N → D | ||||||
Sequence conflict | 8 | in Ref. 1 and 3; AAH68664 | ||||
Sequence: G → E | ||||||
Compositional bias | 15-29 | Acidic residues | ||||
Sequence: QDMEDVEDVEEEETG | ||||||
Sequence conflict | 27-29 | in Ref. 4; AAF86278 | ||||
Sequence: ETG → GTR | ||||||
Sequence conflict | 37 | in Ref. 3; AAH68664 | ||||
Sequence: R → G | ||||||
Sequence conflict | 182 | in Ref. 1 and 4; AAF86278 | ||||
Sequence: S → N | ||||||
Compositional bias | 346-375 | Acidic residues | ||||
Sequence: AIEDDDDDYDEEGEEADDEEGEEEADEDND | ||||||
Sequence conflict | 357 | in Ref. 1 and 4; AAF86278 | ||||
Sequence: E → Q | ||||||
Sequence conflict | 365-368 | in Ref. 1 and 4; AAF86278 | ||||
Sequence: EGEE → FESR | ||||||
Alternative sequence | VSP_052856 | 382-392 | in isoform 2 | |||
Sequence: KGQNPAECKQQ → V |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ020268 EMBL· GenBank· DDBJ | AAY43229.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ020269 EMBL· GenBank· DDBJ | AAY43230.1 EMBL· GenBank· DDBJ | mRNA | ||
BC068664 EMBL· GenBank· DDBJ | AAH68664.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AF278538 EMBL· GenBank· DDBJ | AAF86278.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. |