Q4RSB2 · Q4RSB2_TETNG
- ProteinPhosphoinositide phospholipase C
- GenePLCz1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids551 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score2/5
Function
function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H+This reaction proceeds in the forward direction.
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | phosphatidylinositol phospholipase C activity | |
Biological Process | intracellular signal transduction | |
Biological Process | lipid catabolic process | |
Biological Process | single fertilization |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoinositide phospholipase C
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Neoteleostei > Acanthomorphata > Eupercaria > Tetraodontiformes > Tetradontoidea > Tetraodontidae > Tetraodon
Accessions
- Primary accessionQ4RSB2
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 246-275 | Disordered | ||||
Sequence: KTEAEESVDEGEDEEEEEDEEEEEEEETKK | ||||||
Compositional bias | 252-271 | Acidic residues | ||||
Sequence: SVDEGEDEEEEEDEEEEEEE | ||||||
Domain | 293-408 | PI-PLC Y-box | ||||
Sequence: LSDLVVYTRSVKFCSFTHSRDNQHSYENTSLEENKARKLLKSSGADFVGHNQRFLSRIYPAGSRTSSSNYNPQEFWNVGCQLVALNFQSLAAPMDLNDGRFQDNGGCGYVLKPVVL | ||||||
Domain | 409-533 | C2 | ||||
Sequence: MSSQGCFDPRRSQRRVRPVHLLLKVISGSNLPILKSRKSLDPFVKVEIHGIPSDSSKQSTHTVKNNSLSPHWDAHMNFKISTPELCLIRFCVRDQTGLLSSEFVGQYTLPFTSLMKGYRWVPLWS |
Family and domain databases
Sequence
- Sequence statusFragment
- Length551
- Mass (Da)62,958
- Last updated2005-07-19 v1
- Checksum38CE594D538EA4E0
Features
Showing features for compositional bias, non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 252-271 | Acidic residues | ||||
Sequence: SVDEGEDEEEEEDEEEEEEE | ||||||
Non-terminal residue | 551 | |||||
Sequence: S |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HQ185299 EMBL· GenBank· DDBJ | ADX05669.1 EMBL· GenBank· DDBJ | mRNA | ||
CAAE01015000 EMBL· GenBank· DDBJ | CAG08720.1 EMBL· GenBank· DDBJ | Genomic DNA |