Q4P2U6 · ATG8_USTMA

Function

function

Ubiquitin-like modifier involved in autophagosomes formation. With ATG4, mediates the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).
Required for selective autophagic degradation of the mitochondria (mitophagy) which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Required for wild-type budding of haploid sporidia and for complete symptom development during pathogenic growth such as gall formation and teliospore production in ears of mature maize (PubMed:20618705, PubMed:22843561).

Features

Showing features for site.

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TypeIDPosition(s)Description
Site116-117Cleavage; by ATG4

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentautophagosome membrane
Cellular Componentcytoplasmic vesicle
Cellular Componentfungal-type vacuole membrane
Molecular Functionphosphatidylethanolamine binding
Biological Processautophagosome assembly
Biological Processautophagosome maturation
Biological Processautophagy of mitochondrion
Biological Processcellular response to nitrogen starvation
Biological Processprotein transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Autophagy-related protein 8
  • Alternative names
    • Autophagy-related ubiquitin-like modifier atg8

Gene names

    • Name
      ATG8
    • ORF names
      UMAG_05567

Organism names

Accessions

  • Primary accession
    Q4P2U6
  • Secondary accessions
    • A0A0D1DQB5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Prevents vacuolar accumulation of autophagosomes and affects survival during carbon starvation.

Miscellaneous

PTM/Processing

Features

Showing features for chain, lipidation, propeptide.

TypeIDPosition(s)Description
ChainPRO_00000172381-116
Lipidation116Phosphatidylethanolamine amidated glycine
PropeptidePRO_0000017239117-118Removed in mature form

Post-translational modification

The C-terminal 2 residues are removed by ATG4 to expose Gly-116 at the C-terminus. The C-terminal Gly is then amidated with phosphatidylethanolamine by an activating system similar to that for ubiquitin.

Keywords

Expression

Induction

Transcripts accumulate during carbon stress conditions.

Interaction

Subunit

Conjugation to phosphatidylethanolamine (PE) leads to homodimerization. Interacts with ATG1, ATG3, ATG4, ATG7, ATG12, ATG32, ATG34 and the C-terminal 10 residues domain of ATG19. Interacts also with the endoplasmic reticulum to Golgi v-SNARE protein BET1 and the vacuolar v-SNARE protein NYV1. Interacts with the UBX domain-containing protein SHP1.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the ATG8 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    118
  • Mass (Da)
    13,675
  • Last updated
    2005-07-19 v1
  • Checksum
    8756A51F7BE05C3C
MRSAFKNEHSFEKRKAEAERIRQKYPDRIPVICEKADRTDIPTIDKKKYLVPSDLTVGQFVYVIRKRIKLAPEKAIFIFVDEVLPATAALMSAIYEEHKDEDGFLYVSYSGENTFGQL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM003157
EMBL· GenBank· DDBJ
KIS66579.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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